ProtParam
User-provided sequence:
10 20 30 40 50 60
MRAFPRVLPF RHQRSYNNIL LRTVRLFGSS LSSFDFSRQM PKVDPDNTAA MLLQKNLIQR
70 80 90 100 110 120
NNMLYGYGSG TIRCTLLDST GRAKSPLVEI KREDLVSKHG LLPRDLRKIE KSRKNDLVPS
130 140 150 160 170 180
LLVRENSILI SLLTVKALIK PDMVIIFDSA GSGITLNSEA HKDFINDMKL RLKNQETSEL
190 200 210 220 230 240
NSDPLPYEFR ALETIFISAL SNLTSEMKVL LTICKGVLQD LEFSITRDKL RFLLGQNKKL
250 260 270 280 290 300
SSFNKKAVLV KDMLDDLLEQ DDMLCDMYLT DKKAGKIRVQ DDHTEIEMLL ETYHNYVDEI
310 320 330 340 350 360
VQKSESAISD VKTTEEIINI ILDSNRNELM LLGIRYAIGM LSLGGALFLG SIYGMNLESF
370 380 390 400 410
IEESNYAYLT VTILGLISTV WLYAKGIRHL HKLQRMTLLS KIKTDSVHEL LKK
References and
documentation are available.
Number of amino acids: 413
Molecular weight: 47082.91
Theoretical pI: 8.83
Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 56
Atomic composition:
Carbon C 2100
Hydrogen H 3440
Nitrogen N 566
Oxygen O 618
Sulfur S 18
Formula: C
2100H
3440N
566O
618S
18
Total number of atoms: 6742
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23505
Abs 0.1% (=1 g/l) 0.499, assuming all pairs of Cys residues form cystines
Ext. coefficient 23380
Abs 0.1% (=1 g/l) 0.497, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.95
This classifies the protein as unstable.
Aliphatic index: 110.46
Grand average of hydropathicity (GRAVY): -0.122