ProtParam
User-provided sequence:
10 20 30 40 50 60
MRAFSAATVR ATTRKSFIPM APRTPFVTPS FTKNVGSMRR MRFYSDEAKS EESKENNEDL
70 80 90 100 110 120
TEEQSEIKKL ESQLSAKTKE ASELKDRLLR SVADFRNLQQ VTKKDIQKAK DFALQKFAKD
130 140 150 160 170 180
LLESVDNFGH ALNAFKEEDL QKSKEISDLY TGVRMTRDVF ENTLRKHGIE KLDPLGEPFD
190 200 210 220
PNKHEATFEL PQPDKEPGTV FHVQQLGFTL NDRVIRPAKV GIVKGEEN
References and
documentation are available.
Number of amino acids: 228
Molecular weight: 26066.49
Theoretical pI: 7.86
Total number of negatively charged residues (Asp + Glu): 38
Total number of positively charged residues (Arg + Lys): 39
Atomic composition:
Carbon C 1148
Hydrogen H 1839
Nitrogen N 325
Oxygen O 357
Sulfur S 5
Formula: C
1148H
1839N
325O
357S
5
Total number of atoms: 3674
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 2980
Abs 0.1% (=1 g/l) 0.114
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.36
This classifies the protein as unstable.
Aliphatic index: 68.03
Grand average of hydropathicity (GRAVY): -0.810