ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRAHRIDTFL IRENIKLEII HESNSYFGGE HISIAFRFKH LGSQHELFNY KEKLLTVDKA 

        70         80         90        100        110        120 
VEEKLEQQAK VQDDGEGTME NQTWSLKSLL GAFKRTGEPE ESVDVDNMKM LNESKMLREK 

       130        140        150        160        170        180 
IQKQMYFHQP VTLISGYVQI SGVFQYDSEV ISESKFKQDE VKMVGLDIVP GHTTNSVLAL 

       190        200        210        220        230        240 
EDGEHFKGKR NLTNYLNSDY TNVTNGLLFS ESGSRGRTGT YNERTLMISN DTSIKTLPLL 

       250        260        270        280        290        300 
LIPQTLLFSE ISLEPGEVRT FYFKSTKLPK DICPSYSSSK VASINYTLEV GADVLSDDNI 

       310        320        330        340        350        360 
EKFSNRVPIT IAPYISSNAE QYTSRLDKPA IILKTGNIKE LKPRLFTRKV STASAVSFGR 

       370        380        390        400        410        420 
RKSSIIDIDS PLEDNEFVKR VKKNFIELVE SNQNVSRDID ELIDLQMGVQ FGKDEDSSDP 

       430        440        450        460        470        480 
EPNDSHFSNE MVTSAESSLR SDAVTKRRKS YSVRDNISNL EQKMWNDCSL VKSDENSNLL 

       490        500        510        520        530        540 
PQLINLQNAY QINRNNETMA KVSLSAPFYK TTDDINLVIE LDPITTPLLK VTSLTVSLES 

       550        560        570        580        590        600 
FEIINPKYKT EGKGIGSKPK GNSVYEKHFI CFDECKSVSV KLLPPRSPTN QITGQFKTDV 

       610        620        630        640        650        660 
FQHKWMIGLK FVIIAKTESI TLDQFYEDKK GILFHSKENL EGEEFTCYVP IPILCTSEDF 


MGW 

References and documentation are available.

Number of amino acids: 663

Molecular weight: 75317.40

Theoretical pI: 5.61

Amino acid composition: 
Ala (A)  19	  2.9%
Arg (R)  25	  3.8%
Asn (N)  40	  6.0%
Asp (D)  39	  5.9%
Cys (C)   6	  0.9%
Gln (Q)  24	  3.6%
Glu (E)  55	  8.3%
Gly (G)  32	  4.8%
His (H)  12	  1.8%
Ile (I)  49	  7.4%
Leu (L)  60	  9.0%
Lys (K)  56	  8.4%
Met (M)  14	  2.1%
Phe (F)  32	  4.8%
Pro (P)  26	  3.9%
Ser (S)  68	 10.3%
Thr (T)  42	  6.3%
Trp (W)   4	  0.6%
Tyr (Y)  20	  3.0%
Val (V)  40	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 94
Total number of positively charged residues (Arg + Lys): 81

Atomic composition:

Carbon      C	      3346
Hydrogen    H	      5300
Nitrogen    N	       886
Oxygen      O	      1046
Sulfur      S	        20

Formula: C3346H5300N886O1046S20
Total number of atoms: 10598

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52175
Abs 0.1% (=1 g/l)   0.693, assuming all pairs of Cys residues form cystines


Ext. coefficient    51800
Abs 0.1% (=1 g/l)   0.688, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.19
This classifies the protein as stable.



Aliphatic index: 84.48

Grand average of hydropathicity (GRAVY): -0.465