ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRALNSRLVL IDINSSWQAS RRLISATATA FSSDSSSSFR RTRGARQRIA SSKSPASSPS 

        70         80         90        100        110        120 
PVRRPSDGFS FDVRSPSSDS SISSRKSPTT APPTVELDAF LEILPPATRK ELVKHEAIEE 

       130        140        150        160        170        180 
LIEVVMDLGR KPLARFPSGD WVISEQPVTH QDLELAVSKV GDFSDDNRSG IDRSLHRISA 

       190        200        210        220        230        240 
IRNRKLQVIG LTCRVGRVVS GSAEIIRDLI EGGGSILVIG SPGVGKTTLI REIARMLADE 

       250        260        270        280        290        300 
HRKRVVIVDT SNEIGGDGDV PHSGIGRARR MQVPNVNLQH DVMIEAVENH MPETIIIDEI 

       310        320        330        340        350        360 
GTELEALAAS TIAQRGVQLV ATAHGMTIDN IIKNPSLQIL IGGIESVTLG DEEARKRKVQ 

       370        380        390        400        410        420 
KTILERKGPP TFTCAVEMIS RTECRVHQRL DVTVDAILAG KSAPFEIRQI RGEDDVPHKL 

       430        440        450        460        470        480 
VTPIPLENLE EEPAPLLNRD FVSELLSDDE DEDFLLIRSN KARSNTYTSP RSSPVHVYTY 

       490        500        510        520        530        540 
NVLEADLLQV AEVMGLDDEI EVTDDVGEAD VILASSSELK QNSSIRRVAK LHKLPIFVIK 


VSFFLHG 

References and documentation are available.

Number of amino acids: 547

Molecular weight: 60023.24

Theoretical pI: 5.74

Amino acid composition: 
Ala (A)  38	  6.9%
Arg (R)  46	  8.4%
Asn (N)  16	  2.9%
Asp (D)  37	  6.8%
Cys (C)   3	  0.5%
Gln (Q)  15	  2.7%
Glu (E)  41	  7.5%
Gly (G)  32	  5.9%
His (H)  13	  2.4%
Ile (I)  47	  8.6%
Leu (L)  48	  8.8%
Lys (K)  21	  3.8%
Met (M)   9	  1.6%
Phe (F)  14	  2.6%
Pro (P)  29	  5.3%
Ser (S)  58	 10.6%
Thr (T)  28	  5.1%
Trp (W)   2	  0.4%
Tyr (Y)   3	  0.5%
Val (V)  47	  8.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 67

Atomic composition:

Carbon      C	      2615
Hydrogen    H	      4297
Nitrogen    N	       765
Oxygen      O	       824
Sulfur      S	        12

Formula: C2615H4297N765O824S12
Total number of atoms: 8513

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    15595
Abs 0.1% (=1 g/l)   0.260, assuming all pairs of Cys residues form cystines


Ext. coefficient    15470
Abs 0.1% (=1 g/l)   0.258, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 59.45
This classifies the protein as unstable.



Aliphatic index: 99.60

Grand average of hydropathicity (GRAVY): -0.218