ProtParam
User-provided sequence:
10 20 30 40 50 60
MRALRFLIEN RNTVFFTLLV ALVLSLYLLV YLFSHTPRPP YPEELKYIAI DEKGHEVSRA
70 80 90 100 110 120
LPNLNEHQDD EEIFLSVVIP SYNETGRILL MLTDAISFLK EKYGSRWEIV IVDDGSTDNT
130 140 150 160 170 180
TQYCLKICKE QFKLNYEQFR IIKFSQNRGK GGAVRQGFLH IRGKYGLFAD ADGASKFSDV
190 200 210 220 230 240
EKLIDAISKI ETSSTDLKTT KPAVAIGSRA HMVNTEAVIK RSMIRNCLMY GFHTLVFIFG
250 260 270 280 290 300
IRSIKDTQCG FKLFNRAAIL KIFPYLHTEG WIFDVEILIL AIRKRIQIEE IPISWHEVDG
310 320 330
SKMALAIDSI KMAKDLVIIR MAYLLGIYRD NKKC
References and
documentation are available.
Number of amino acids: 334
Molecular weight: 38346.82
Theoretical pI: 8.90
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 45
Atomic composition:
Carbon C 1749
Hydrogen H 2774
Nitrogen N 458
Oxygen O 482
Sulfur S 13
Formula: C
1749H
2774N
458O
482S
13
Total number of atoms: 5476
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36120
Abs 0.1% (=1 g/l) 0.942, assuming all pairs of Cys residues form cystines
Ext. coefficient 35870
Abs 0.1% (=1 g/l) 0.935, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.30
This classifies the protein as stable.
Aliphatic index: 105.99
Grand average of hydropathicity (GRAVY): 0.017