ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRAPPSPRKS KSGHFFYLYF RLCQLFSGRK LKRRWHVHKL HIHQYNTRWN LSPLSEIHIE 

        70         80         90        100        110        120 
DMINEPSGLC PGSSKKKPLL IARFPKGCQE SPRVYVLQRN NLSRLKLSKR KYALRFYHNE 

       130        140        150        160        170        180 
IFGNNLKRKD GSIHKVEHQQ CAETVRKIKK VTANHADVKI IFHDKNTIRS DKLGGRSNKM 

       190        200        210        220        230        240 
QTRPSVLEED VEEEVSSVYI RFCDDHSLRV KDYHSLHRHS KKSSKEKRNN QEIGKSKLLG 

       250        260        270 
KLFEEETSRQ NKGVEKKLDT IVIQKFQNYP IVSFSRVI 

References and documentation are available.

Number of amino acids: 278

Molecular weight: 32757.80

Theoretical pI: 10.10

Amino acid composition: 
Ala (A)   6	  2.2%
Arg (R)  24	  8.6%
Asn (N)  15	  5.4%
Asp (D)  10	  3.6%
Cys (C)   5	  1.8%
Gln (Q)  11	  4.0%
Glu (E)  18	  6.5%
Gly (G)  12	  4.3%
His (H)  15	  5.4%
Ile (I)  17	  6.1%
Leu (L)  24	  8.6%
Lys (K)  34	 12.2%
Met (M)   3	  1.1%
Phe (F)  12	  4.3%
Pro (P)  11	  4.0%
Ser (S)  27	  9.7%
Thr (T)   7	  2.5%
Trp (W)   2	  0.7%
Tyr (Y)   9	  3.2%
Val (V)  16	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 58

Atomic composition:

Carbon      C	      1456
Hydrogen    H	      2340
Nitrogen    N	       442
Oxygen      O	       404
Sulfur      S	         8

Formula: C1456H2340N442O404S8
Total number of atoms: 4650

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    24660
Abs 0.1% (=1 g/l)   0.753, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.745, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 62.02
This classifies the protein as unstable.



Aliphatic index: 76.37

Grand average of hydropathicity (GRAVY): -0.872