ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRAWTGSWRW IMLILFAWGT LLFYIGGHLV RDNDHPDHSS RELSKILAKL ERLKQQNEDL 

        70         80         90        100        110        120 
RRMAESLRIP EGPIDQGTAT GRVRVLEEQL VKAKEQIENY KKQARNGLGK DHELLRRRIE 

       130        140        150        160        170        180 
NGAKELWFFL QSELKKLKHL EGNELQRHAD EILLDLGHHE RSIMTDLYYL SQTDGAGDWR 

       190        200        210        220        230        240 
EKEAKDLTEL VQRRITYLQN PKDCSKARKL VCNINKGCGY GCQLHHVVYC FMIAYGTQRT 

       250        260        270        280        290        300 
LILESQNWRY ATGGWETVFR PVSETCTDRS GLSTGHWSGE VNDKNIQVVE LPIVDSLHPR 

       310        320        330        340        350        360 
PPYLPLAVPE DLADRLVRVH GDPAVWWVSQ FVKYLIRPQP WLEKEIEEAT KKLGFKHPVI 

       370        380        390        400        410        420 
GVHVRRTDKV GTEAAFHPIE EYMVHVEEHF QLLARRMQVD KKRVYLATDD PALLKEAKTK 

       430        440        450        460        470        480 
YSNYEFISDN SISWSAGLHN RYTENSLRGV ILDIHFLSQA DFLVCTFSSQ VCRVAYEIMQ 

       490        500        510        520        530        540 
TLHPDASANF HSLDDIYYFG GQNAHNQIAV YPHKPRTDEE IPMEPGDIIG VAGNHWDGYS 

       550        560        570 
KGVNRKLGKT GLYPSYKVRE KIETVKYPTY PEAEK 

References and documentation are available.

Number of amino acids: 575

Molecular weight: 66484.68

Theoretical pI: 7.13

Amino acid composition: 
Ala (A)  33	  5.7%
Arg (R)  39	  6.8%
Asn (N)  21	  3.7%
Asp (D)  32	  5.6%
Cys (C)   8	  1.4%
Gln (Q)  24	  4.2%
Glu (E)  46	  8.0%
Gly (G)  38	  6.6%
His (H)  25	  4.3%
Ile (I)  31	  5.4%
Leu (L)  58	 10.1%
Lys (K)  38	  6.6%
Met (M)   9	  1.6%
Phe (F)  16	  2.8%
Pro (P)  25	  4.3%
Ser (S)  29	  5.0%
Thr (T)  27	  4.7%
Trp (W)  14	  2.4%
Tyr (Y)  25	  4.3%
Val (V)  37	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 77

Atomic composition:

Carbon      C	      2980
Hydrogen    H	      4631
Nitrogen    N	       839
Oxygen      O	       858
Sulfur      S	        17

Formula: C2980H4631N839O858S17
Total number of atoms: 9325

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   114750
Abs 0.1% (=1 g/l)   1.726, assuming all pairs of Cys residues form cystines


Ext. coefficient   114250
Abs 0.1% (=1 g/l)   1.718, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.39
This classifies the protein as unstable.



Aliphatic index: 84.77

Grand average of hydropathicity (GRAVY): -0.557