ProtParam
User-provided sequence:
10 20 30 40 50 60
MRDDRERGEG DLSWPLIGEK SSVKEEVKKQ LWLSGPLIAV SLLQFCLQVI SVMFVGHLGS
70 80 90 100 110 120
LPLSAASIAT SFASVTGFSF LMGTASALDT LCGQAYGAKK YGMLGIQMQR AMFVLTLASI
130 140 150 160 170 180
PLSIIWANTE HLLVFFGQNK SIATLAGSYA KFMIPSIFAY GLLQCFNRFL QAQNNVFPVV
190 200 210 220 230 240
FCSGVTTSLH VLLCWVLVFK SGLGFQGAAL ANSISYWLNV VLLFCYVKFS PSCSLTWTGF
250 260 270 280 290 300
SKEALRDILP FLRLAVPSAL MVCLEMWSFE LLVLLSGLLP NPVLETSVLS ICLNTSGTMW
310 320 330 340 350 360
MIPFGLSGAA STRISNELGA GNPKVAKLAV RVVICIAVAE SIVIGSVLIL IRNIWGLAYS
370 380 390 400 410 420
SELEVVSYVA SMMPILALGN FLDSLQCVLS GVARGCGWQK IGAIINLGSY YLVGVPSGLL
430 440 450 460 470 480
LAFHFHVGGR GLWLGIICAL VVQVFGLGLV TIFTNWDEEA KKATNRIESS SSVKDFAVDD
RSVVVF
References and
documentation are available.
Number of amino acids: 486
Molecular weight: 52472.98
Theoretical pI: 8.37
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 31
Atomic composition:
Carbon C 2418
Hydrogen H 3805
Nitrogen N 597
Oxygen O 650
Sulfur S 26
Formula: C
2418H
3805N
597O
650S
26
Total number of atoms: 7496
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 81650
Abs 0.1% (=1 g/l) 1.556, assuming all pairs of Cys residues form cystines
Ext. coefficient 80900
Abs 0.1% (=1 g/l) 1.542, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 25.74
This classifies the protein as stable.
Aliphatic index: 118.72
Grand average of hydropathicity (GRAVY): 0.738