ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRDEATRKKR SFSDGHFFKK LKLMSRKKQP VMERSKTTRT RKESTNSAAK SSLSLRRANN 

        70         80         90        100        110        120 
GRKTIAKRRV LTDIGSTNEG VAGNSGSNSP AQYSHTPHFS DSIPPLPLEL PDIVSIRSSR 

       130        140        150        160        170        180 
SHISNKSNKN KHGIDLTFIP RRSLQNSKAG LKKPNTSPQG YFNIPVTIDR ASEKVKHTDT 

       190        200        210        220        230        240 
KNTFNSSSSE NERPVLSILQ KDDSQSSSHP AIDSMSAPNN INNNNDIENS SNSLFDTILS 

       250        260        270        280        290        300 
IAHSAISHVP KISALNTEIQ REFSHSGESH TGSTRHPYFH IHHAQQQHPL SQQQGPLPVS 

       310        320        330        340        350        360 
ENANQNPNDT VLIHSPSANT AHRSSSFLRH LDYLLSPTSG PASDKHTQVE EGDDEEELSP 

       370        380        390        400        410        420 
LSKAFLSPST QLVPTNTSTT PLSGSLTPNN RNVNANSNSE TENDNDRDDR SNVGKVKFQP 

       430        440        450        460        470        480 
LKVHEPAIST FGKGNLTLEA VAGSSDIDNT TIDLDENNTN NNPNASSTNL SHISKSNVNN 

       490        500        510        520        530        540 
NLGPKELNTS YRNSTYIDMA RFENSQSNLS SHRARSKTLP ANKALENAVG DEGNSKRNSR 

       550        560        570        580        590        600 
YSSYSNDMAF DDADERKFRS MSKKFLNRRS FSPSNLGNKV IPGINLRNSF NKNRNSSSDF 

       610        620        630        640        650        660 
FSTNQGQQMP RTSTAGSGNI HAIMGLDSGN DDFKLEGIEY ASEKKNSEFH TLFKDCDINP 

       670        680        690        700        710        720 
NEKLIVDHSC ALSRDILLQG RMYISDAHIG FFSNILGWVS TVFIPFKEIV QIEKKTTAGI 

       730        740        750        760        770        780 
FPNGIVIDTL HTKYIFASFM SRDATFDLIT DVWNQIILGK KYRNGFGNND DGTISDSSSA 

       790        800        810        820        830        840 
FFDDSDDNDD DGDLDDDDPD INSTDMTSSD DIDADVFNES NDLGKNQKST NYLLGPNKHS 

       850        860        870        880        890        900 
PTTADFKPSN NDHLVIEANI NAPLGKVVNL LYGEDVSYYE RILKAQKNFE ISPIPNNFLT 

       910        920        930        940        950        960 
KKIRDYAYTK PLSGSIGPSK TKCLITDTLE HYDLEDYVKV LSITKNPDVP SGNIFSVKTV 

       970        980        990       1000       1010       1020 
FLFSWDKNNS TKLTVYNSVD WTGKSWIKSM IEKGTFDGVA DTTKIMISEI KKILSDEDSN 

      1030       1040       1050       1060       1070       1080 
INSKHQASNN ESEEEIINLP TIGPPVHDPT EPDFQKGKDD TVIDEKINIP VPLGTVFSLL 

      1090       1100       1110       1120       1130       1140 
YGDDTSYIKK IIENQNNFNV CDIPKFVNNA REITYTKKLN NSFGPKQTKC IVTETIEHMD 

      1150       1160       1170       1180       1190       1200 
LNSFFMVKQI VRSPDVPYGS SFSVHTRFFY SWGDHNTTNM KVVTNVVWTG KSMLKGTIEK 

      1210       1220       1230       1240       1250       1260 
GSIDGQRSST KQLVDDLKKI ISNASSTKKK SRRRGKTVNK RKSSPSTIKN EKNEENFEDT 

      1270       1280       1290       1300       1310       1320 
STKNSFFSAF SMLQQVNITS VQGIMTIISF FICLIFFFRL LFHSKNTSNI QIITPGTILI 

      1330       1340       1350       1360       1370       1380 
NGNEYNYVPN FKTLYHVYED NIIKDARRKD SNKNNIVTDT EGLIWDWLID RGNGTVQNSV 

      1390       1400       1410       1420       1430 
LSNHIKESNN KKVKLVNGVS DHKIQQLVES IKITELQLQE MKELLAQTDN TSATNQLL 

References and documentation are available.

Number of amino acids: 1438

Molecular weight: 160578.47

Theoretical pI: 7.01

Amino acid composition: 
Ala (A)  55	  3.8%
Arg (R)  56	  3.9%
Asn (N) 144	 10.0%
Asp (D) 104	  7.2%
Cys (C)   6	  0.4%
Gln (Q)  44	  3.1%
Glu (E)  68	  4.7%
Gly (G)  69	  4.8%
His (H)  40	  2.8%
Ile (I) 106	  7.4%
Leu (L)  97	  6.7%
Lys (K) 113	  7.9%
Met (M)  21	  1.5%
Phe (F)  65	  4.5%
Pro (P)  64	  4.5%
Ser (S) 176	 12.2%
Thr (T) 104	  7.2%
Trp (W)   9	  0.6%
Tyr (Y)  30	  2.1%
Val (V)  67	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 172
Total number of positively charged residues (Arg + Lys): 169

Atomic composition:

Carbon      C	      7003
Hydrogen    H	     11060
Nitrogen    N	      1996
Oxygen      O	      2281
Sulfur      S	        27

Formula: C7003H11060N1996O2281S27
Total number of atoms: 22367

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    94575
Abs 0.1% (=1 g/l)   0.589, assuming all pairs of Cys residues form cystines


Ext. coefficient    94200
Abs 0.1% (=1 g/l)   0.587, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 43.09
This classifies the protein as unstable.



Aliphatic index: 72.39

Grand average of hydropathicity (GRAVY): -0.701