ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRDVPKSLGL SVTTPGGSSG APDSGRHNSL EEINLDQFLK TSNYEDTVKQ LDIYYGIVKR 

        70         80         90        100        110        120 
QLLRYQSPIT GLFPVMSTDQ VVGSVRDSVY CASAVWSLYQ AYRRIDDDRG KSYELGQSTV 

       130        140        150        160        170        180 
KCMRGILECW VKQASRVELF KQRQSNQHAL HSKFQLHTGE KIYPDEFYNH LQIDCVSLYL 

       190        200        210        220        230        240 
LFLVQMITSG LQIIYTHDEV AFVQNLVYYV ERAYRTPDFG MWERGSKYNN GTPEIHASSI 

       250        260        270        280        290        300 
GMAKSALEAI NGCNLFGEKG ASWSVVYVDI DAHNRNRSIF ETMLPRESSS KGVDASLLLT 

       310        320        330        340        350        360 
LSFPAFASHE DRLVEQTKQN VVNRLRCKMG FKRFTRDGFL SKNEDKSRRY YHSGELKEFE 

       370        380        390        400        410        420 
GLECEWPLFF IAMIIDGVFK NNNEQIEEFQ NDLRRCLRTD VNGDPVVTMY YAPDGDGSYM 

       430        440        450        460        470        480 
RAPSQSLFLW GQSFFIIAQL LTAGLLHINE LDPIRRYLPS YNRPRRAGRY SAFQGTATDL 

       490        500        510        520        530        540 
VVQIVLIAES MRLQAMMATY GIQTQTPHEV EPVQIWSSTE LIKVYQHLGV NNKVGLSGRP 

       550        560        570        580        590        600 
CRPVGSLGTS KVYRICGMTV LCYPLIFEVS DFYLYRDMAL LIDDIKTELQ FVGKYWRLSG 

       610        620        630        640        650        660 
RPTVCLLIRE EHMRDPQFKE MLDLLAMLKK GYCDGMKVRI GRLQNLISSS CIEHLDFMNQ 

       670        680        690        700        710        720 
SDLTDNENAF SQINHEYIGY QSLTDVPKAL TYVEEKISVA HFDTKPTPDI INALRSTDSI 

       730        740        750        760        770        780 
YCLCQLWGII LNREGPHFEV NGLNVNTALT QLYHRAGSLR YWRAVRYCSS LLHHIVDSIS 

       790        800        810        820        830        840 
PFITTVLVNG KELTVGIIGQ KETVFDKPMT PAEIQNVMYT SVQPYDVIQA VLQQEVVLYC 

       850        860        870        880        890        900 
GRLIATNPSM FRGILKIRIG WVLEAMRIYL QISGQQSIDV DNLSPFQVRI LLQKVLTVSE 

       910        920        930        940        950        960 
WAVEEKLTTL QRRQLEGCLC RVPKHFYNKI WEILQRTPQG ILTQGHHLPA TPTLTNMSRG 

       970        980        990       1000       1010       1020 
ELTFNLLVEE TLICIDRPER RQITVELLCI VATILNRNPE LHFKQALDLD GILAEAFAMY 

      1030       1040       1050       1060       1070       1080 
CKDNNIQHQP KPEHEQTKNE DLKAFYSLPY SETTGYLARA AVNKVLQGGI FSTNEEDVQL 

      1090 
DGDRLHDDNC KVS 

References and documentation are available.

Number of amino acids: 1093

Molecular weight: 124863.99

Theoretical pI: 6.36

Amino acid composition: 
Ala (A)  52	  4.8%
Arg (R)  67	  6.1%
Asn (N)  49	  4.5%
Asp (D)  57	  5.2%
Cys (C)  24	  2.2%
Gln (Q)  61	  5.6%
Glu (E)  67	  6.1%
Gly (G)  66	  6.0%
His (H)  28	  2.6%
Ile (I)  71	  6.5%
Leu (L) 118	 10.8%
Lys (K)  48	  4.4%
Met (M)  27	  2.5%
Phe (F)  42	  3.8%
Pro (P)  43	  3.9%
Ser (S)  75	  6.9%
Thr (T)  60	  5.5%
Trp (W)  13	  1.2%
Tyr (Y)  49	  4.5%
Val (V)  76	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 124
Total number of positively charged residues (Arg + Lys): 115

Atomic composition:

Carbon      C	      5573
Hydrogen    H	      8743
Nitrogen    N	      1521
Oxygen      O	      1636
Sulfur      S	        51

Formula: C5573H8743N1521O1636S51
Total number of atoms: 17524

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   146010
Abs 0.1% (=1 g/l)   1.169, assuming all pairs of Cys residues form cystines


Ext. coefficient   144510
Abs 0.1% (=1 g/l)   1.157, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.41
This classifies the protein as unstable.



Aliphatic index: 92.36

Grand average of hydropathicity (GRAVY): -0.238