ProtParam
User-provided sequence:
10 20 30 40 50 60
MREESRSRKR KHIPVDIEEV EVRSKYFKKN ERTVELVKEN KINKDLQNYG GVNIDWIKAL
70 80 90 100 110 120
KPIEYFEWIE SRTCDDPRTW GRPITKEEMI NDSGAKVPES FLPIYNRVRL MRSKVKTPVD
130 140 150 160 170 180
AMGCSMIPVL VSNKCGIPSE KVDPKNFRLQ FLIGTMLSAQ TRDERMAQAA LNITEYCLNT
190 200 210 220 230 240
LKIAEGITLD GLLKIDEPVL ANLIRCVSFY TRKANFIKRT AQLLVDNFDS DIPYDIEGIL
250 260 270 280 290 300
SLPGVGPKMG YLTLQKGWGL IAGICVDVHV HRLCKMWNWV DPIKCKTAEH TRKELQVWLP
310 320 330 340 350 360
HSLWYEINTV LVGFGQLICM ARGKRCDLCL ANDVCNARNE KLIESSKFHQ LEDKEDIEKV
370 380
YSHWLDTVTN GITTERHKKK
References and
documentation are available.
Number of amino acids: 380
Molecular weight: 43843.94
Theoretical pI: 8.78
Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 59
Atomic composition:
Carbon C 1952
Hydrogen H 3131
Nitrogen N 541
Oxygen O 560
Sulfur S 22
Formula: C
1952H
3131N
541O
560S
22
Total number of atoms: 6206
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 65150
Abs 0.1% (=1 g/l) 1.486, assuming all pairs of Cys residues form cystines
Ext. coefficient 64400
Abs 0.1% (=1 g/l) 1.469, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 42.45
This classifies the protein as unstable.
Aliphatic index: 91.53
Grand average of hydropathicity (GRAVY): -0.384