ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MREILHIQGG QCGNQIGAKF WEVICGEHGI DQTGQSCGDT DLQLERINVY FNEASGGKYV 

        70         80         90        100        110        120 
PRAVLMDLEP GTMDSLRSGP FGQIFRPDNF VFGQSGAGNN WAKGHYTEGA ELIDSVLDVV 

       130        140        150        160        170        180 
RKEAENCDCL QGFQVCHSLG GGTGSGMGTL LISKIREEYP DRMMMTFSVF PSPKVSDTVV 

       190        200        210        220        230        240 
EPYNATLSVH QLVENADECM VLDNEALYDI CFRTLKLANP TFGDLNHLIS ATMSGVTCCL 

       250        260        270        280        290        300 
RFPGQLNSDL RKLAVNLIPF PRLHFFMVGF APLTSRGSQQ YSALSVPELT QQMWDAKNMM 

       310        320        330        340        350        360 
CAADPRHGRY LTASAVFRGK MSTKEVDEQM MNVQNKNSSY FVEWIPNNVK SSVCDIAPTG 

       370        380        390        400        410        420 
LKMASTFIGN STSIQEMFRR VSEQFTAMFR RKAFLHWYTG EGMDEMEFTE AESNMNDLVA 

       430        440 
EYQQYQDATV GEEEYEEDEE EEEA 

References and documentation are available.

Number of amino acids: 444

Molecular weight: 49657.92

Theoretical pI: 4.69

Amino acid composition: 
Ala (A)  28	  6.3%
Arg (R)  20	  4.5%
Asn (N)  23	  5.2%
Asp (D)  24	  5.4%
Cys (C)  12	  2.7%
Gln (Q)  23	  5.2%
Glu (E)  38	  8.6%
Gly (G)  37	  8.3%
His (H)   9	  2.0%
Ile (I)  17	  3.8%
Leu (L)  33	  7.4%
Lys (K)  15	  3.4%
Met (M)  22	  5.0%
Phe (F)  24	  5.4%
Pro (P)  17	  3.8%
Ser (S)  31	  7.0%
Thr (T)  24	  5.4%
Trp (W)   5	  1.1%
Tyr (Y)  13	  2.9%
Val (V)  29	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 35

Atomic composition:

Carbon      C	      2168
Hydrogen    H	      3338
Nitrogen    N	       588
Oxygen      O	       683
Sulfur      S	        34

Formula: C2168H3338N588O683S34
Total number of atoms: 6811

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    47620
Abs 0.1% (=1 g/l)   0.959, assuming all pairs of Cys residues form cystines


Ext. coefficient    46870
Abs 0.1% (=1 g/l)   0.944, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.86
This classifies the protein as unstable.



Aliphatic index: 69.17

Grand average of hydropathicity (GRAVY): -0.332