ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRFFLFPYER FQLHQPLFLF FSICFLTLRM KERLLRYNLR YLYALRNVFG TFYSNQRSNQ 

        70 
NVATLSKEPF LVFSF 

References and documentation are available.

Number of amino acids: 75

Molecular weight: 9336.99

Theoretical pI: 10.36

Amino acid composition: 
Ala (A)   2	  2.7%
Arg (R)   8	 10.7%
Asn (N)   5	  6.7%
Asp (D)   0	  0.0%
Cys (C)   1	  1.3%
Gln (Q)   4	  5.3%
Glu (E)   3	  4.0%
Gly (G)   1	  1.3%
His (H)   1	  1.3%
Ile (I)   1	  1.3%
Leu (L)  13	 17.3%
Lys (K)   2	  2.7%
Met (M)   2	  2.7%
Phe (F)  13	 17.3%
Pro (P)   3	  4.0%
Ser (S)   5	  6.7%
Thr (T)   3	  4.0%
Trp (W)   0	  0.0%
Tyr (Y)   5	  6.7%
Val (V)   3	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 3
Total number of positively charged residues (Arg + Lys): 10

Atomic composition:

Carbon      C	       445
Hydrogen    H	       658
Nitrogen    N	       112
Oxygen      O	       104
Sulfur      S	         3

Formula: C445H658N112O104S3
Total number of atoms: 1322

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     7450
Abs 0.1% (=1 g/l)   0.798, assuming all pairs of Cys residues form cystines


Ext. coefficient     7450
Abs 0.1% (=1 g/l)   0.798, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.90
This classifies the protein as stable.



Aliphatic index: 87.07

Grand average of hydropathicity (GRAVY): 0.080