ProtParam
User-provided sequence:
10 20 30 40 50 60
MRFLSKRILK PVLSVIILIS IAVTVVLYFL TANENYLQAV KDSAKSQYAS LRESYKSITG
70 80 90 100 110 120
KTESADELPD HDAEVLDSIM DRLHEPLYEK DTFDPNEVLA ENKQLYEEFL LQEISEPKVD
130 140 150 160 170 180
NLVRSGDPLA GKAKGTILSL VRNSDLEDII SSIQQLEEEY NKNFGYPYTF LNDEEFTDEF
190 200 210 220 230 240
KDGIKSILPK DRVVEFGTIG PDNWNMPDSI DRERYDQEMD KMSKENIQYA EVESYHNMCR
250 260 270 280 290 300
FYSKEFYHHP LLSKYKYVWR LEPNVNFYCK INYDVFQFMN KNDKIYGFVL NLYDSPQTIE
310 320 330 340 350 360
TLWTSTMDFV EEHPNYLNVN GAFAWLKDNS QNPKNYDYTQ GYSTCHFWTN FEIVDLDFLR
370 380 390 400 410 420
SEPYEKYMQY LEEKGGFYYE RWGDAPVRSL ALALFADKSS IHWFRDIGYH HTPYTNCPTC
430 440 450 460
PADSDRCNGN CVPGKFTPWS DLDNQNCQAT WIRHSMSEEE LEMY
References and
documentation are available.
Number of amino acids: 464
Molecular weight: 54555.99
Theoretical pI: 4.72
Total number of negatively charged residues (Asp + Glu): 77
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 2458
Hydrogen H 3665
Nitrogen N 621
Oxygen O 752
Sulfur S 19
Formula: C
2458H
3665N
621O
752S
19
Total number of atoms: 7515
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 97680
Abs 0.1% (=1 g/l) 1.790, assuming all pairs of Cys residues form cystines
Ext. coefficient 97180
Abs 0.1% (=1 g/l) 1.781, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.56
This classifies the protein as unstable.
Aliphatic index: 72.26
Grand average of hydropathicity (GRAVY): -0.625