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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRFSTTLATA ATALFFTASQ VSAIGELAFN LGVKNNDGTC KSTSDYETEL QALKSYTSTV

70 80 90 100 110 120
KVYAASDCNT LQNLGPAAEA EGFTIFVGVW PTDDSHYAAE KAALQTYLPK IKESTVAGFL

130 140 150 160 170 180
VGSEALYRND LTASQLSDKI NDVRSVVADI SDSDGKSYSG KQVGTVDSWN VLVAGYNSAV

190 200 210 220 230 240
IEASDFVMAN AFSYWQGQTM QNASYSFFDD IMQALQVIQS TKGSTDITFW VGETGWPTDG

250 260 270 280 290 300
TNFESSYPSV DNAKQFWKEG ICSMRAWGVN VIVFEAFDED WKPNTSGTSD VEKHWGVFTS

310
SDNLKYSLDC DFS


References and documentation are available.

Number of amino acids: 313 Molecular weight: 34118.60 Theoretical pI: 4.32
Amino acid composition: 
Ala (A) 31 9.9% Arg (R) 4 1.3% Asn (N) 16 5.1% Asp (D) 24 7.7% Cys (C) 4 1.3% Gln (Q) 13 4.2% Glu (E) 15 4.8% Gly (G) 21 6.7% His (H) 2 0.6% Ile (I) 11 3.5% Leu (L) 18 5.8% Lys (K) 16 5.1% Met (M) 5 1.6% Phe (F) 18 5.8% Pro (P) 6 1.9% Ser (S) 36 11.5% Thr (T) 27 8.6% Trp (W) 9 2.9% Tyr (Y) 12 3.8% Val (V) 25 8.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 39 Total number of positively charged residues (Arg + Lys): 20 Atomic composition: Carbon C 1518 Hydrogen H 2279 Nitrogen N 383 Oxygen O 496 Sulfur S 9 Formula: C1518H2279N383O496S9 Total number of atoms: 4685 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 67630 Abs 0.1% (=1 g/l) 1.982, assuming all pairs of Cys residues form cystines Ext. coefficient 67380 Abs 0.1% (=1 g/l) 1.975, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 21.11 This classifies the protein as stable. Aliphatic index: 69.20 Grand average of hydropathicity (GRAVY): -0.210