ProtParam
User-provided sequence:
10 20 30 40 50 60
MRFSTTLATA ATALFFTASQ VSAIGELAFN LGVKNNDGTC KSTSDYETEL QALKSYTSTV
70 80 90 100 110 120
KVYAASDCNT LQNLGPAAEA EGFTIFVGVW PTDDSHYAAE KAALQTYLPK IKESTVAGFL
130 140 150 160 170 180
VGSEALYRND LTASQLSDKI NDVRSVVADI SDSDGKSYSG KQVGTVDSWN VLVAGYNSAV
190 200 210 220 230 240
IEASDFVMAN AFSYWQGQTM QNASYSFFDD IMQALQVIQS TKGSTDITFW VGETGWPTDG
250 260 270 280 290 300
TNFESSYPSV DNAKQFWKEG ICSMRAWGVN VIVFEAFDED WKPNTSGTSD VEKHWGVFTS
310
SDNLKYSLDC DFS
References and
documentation are available.
Number of amino acids: 313
Molecular weight: 34118.60
Theoretical pI: 4.32
Total number of negatively charged residues (Asp + Glu): 39
Total number of positively charged residues (Arg + Lys): 20
Atomic composition:
Carbon C 1518
Hydrogen H 2279
Nitrogen N 383
Oxygen O 496
Sulfur S 9
Formula: C
1518H
2279N
383O
496S
9
Total number of atoms: 4685
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 67630
Abs 0.1% (=1 g/l) 1.982, assuming all pairs of Cys residues form cystines
Ext. coefficient 67380
Abs 0.1% (=1 g/l) 1.975, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 21.11
This classifies the protein as stable.
Aliphatic index: 69.20
Grand average of hydropathicity (GRAVY): -0.210