ProtParam
User-provided sequence:
10 20 30 40 50 60
MRGEIDEANL APETSLINKE NQDSSATITT TLLLTTFVAV SGSFVFGSAI GYSSPVQSDL
70 80 90 100 110 120
TKELNLSVAE YSLFGSILTI GAMIGAAMSG RIADMIGRRA TMGFSEMFCI LGWLAIYLSK
130 140 150 160 170 180
VAIWLDVGRF LVGYGMGVFS FVVPVYIAEI TPKGLRGGFT TVHQLLICLG VSVTYLLGSF
190 200 210 220 230 240
IGWRILALIG MIPCVVQMMG LFVIPESPRW LAKVGKWEEF EIALQRLRGE SADISYESNE
250 260 270 280 290 300
IKDYTRRLTD LSEGSIVDLF QPQYAKSLVV GVGLMVLQQF GGVNGIAFYA SSIFESAGVS
310 320 330 340 350 360
SKIGMIAMVV VQIPMTTLGV LLMDKSGRRP LLLISATGTC IGCFLVGLSF SLQFVKQLSG
370 380 390 400 410 420
DASYLALTGV LVYTGSFSLG MGGIPWVIMS EIFPIDIKGS AGSLVTVVSW VGSWIISFTF
430 440 450 460 470
NFLMNWNPAG TFYVFATVCG ATVIFVAKLV PETKGRTLEE IQYSIGYVEL
References and
documentation are available.
Number of amino acids: 470
Molecular weight: 50739.53
Theoretical pI: 5.20
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 2332
Hydrogen H 3660
Nitrogen N 562
Oxygen O 650
Sulfur S 24
Formula: C
2332H
3660N
562O
650S
24
Total number of atoms: 7228
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 72225
Abs 0.1% (=1 g/l) 1.423, assuming all pairs of Cys residues form cystines
Ext. coefficient 71850
Abs 0.1% (=1 g/l) 1.416, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 33.33
This classifies the protein as stable.
Aliphatic index: 111.53
Grand average of hydropathicity (GRAVY): 0.652