ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRGKVKERHV GFKGSAILKQ HRCNWNIKRL NMIPRTRTLL QSKIPITRYF ARCWAPRVRY 

        70         80         90        100        110        120 
NVCRTLPAAA LHTNIIAHNE VKKDDKKVHL GSFKVDKPKM MIAFTCKKCN TRSSHTMSKQ 

       130        140        150        160        170        180 
AYEKGTVLIS CPHCKVRHLI ADHLKIFHDH HVTVEQLMKA NGEQVSQDVG DLEFEDIPDS 

       190        200 
LKDVLGKYAK NNSENASQLP HPSQK 

References and documentation are available.

Number of amino acids: 205

Molecular weight: 23542.42

Theoretical pI: 9.86

Amino acid composition: 
Ala (A)  13	  6.3%
Arg (R)  13	  6.3%
Asn (N)  11	  5.4%
Asp (D)  10	  4.9%
Cys (C)   7	  3.4%
Gln (Q)   8	  3.9%
Glu (E)   8	  3.9%
Gly (G)   8	  3.9%
His (H)  13	  6.3%
Ile (I)  12	  5.9%
Leu (L)  15	  7.3%
Lys (K)  24	 11.7%
Met (M)   6	  2.9%
Phe (F)   6	  2.9%
Pro (P)   9	  4.4%
Ser (S)  12	  5.9%
Thr (T)  10	  4.9%
Trp (W)   2	  1.0%
Tyr (Y)   4	  2.0%
Val (V)  14	  6.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 18
Total number of positively charged residues (Arg + Lys): 37

Atomic composition:

Carbon      C	      1035
Hydrogen    H	      1677
Nitrogen    N	       315
Oxygen      O	       287
Sulfur      S	        13

Formula: C1035H1677N315O287S13
Total number of atoms: 3327

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    17335
Abs 0.1% (=1 g/l)   0.736, assuming all pairs of Cys residues form cystines


Ext. coefficient    16960
Abs 0.1% (=1 g/l)   0.720, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.02
This classifies the protein as stable.



Aliphatic index: 77.51

Grand average of hydropathicity (GRAVY): -0.612