ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRGMSYNITH ECDAINILSD NLHEGAISED MVALSGPAIE LLENNVGSSK NSYQEDEGSS 

        70         80         90        100        110        120 
SIDENAPLIS IKRKRRIRTV KSTSNKELVQ RKASKPTKQK SVFTPLEQQY LELKKNYQET 

       130        140        150        160        170        180 
ILAIEVGYKF RFFGKDAKIA SEVLGISCYF EHNFLNASVP SYRIDYHLER LINFGLKVAV 

       190        200        210        220        230        240 
VRQTETAALK STSSSRNTLF DRRVARVLTK GTTLDDSFFR FEQTQHGTLQ ASQFILCVAD 

       250        260        270        280        290        300 
NVDKSKAKSG RVQVGLIAIQ LSSGTTVYDH FQDDFLRSEL QTRLSHFQPC ELIYSNKLSS 

       310        320        330        340        350        360 
ESVALLNHYV STEKTCGRVV RVQHAVQQDV KLALENLQDF FSSKCIMSGS KIIELHMEKV 

       370        380        390        400        410        420 
KSLHSLSIIC LDMAISYLME FSLEDLFVAS NFYQPFDSIS SMVLSKQALE GLELFVNQTD 

       430        440        450        460        470        480 
HTPVGSLFWV LDRTYTRFGQ RMLQRWLQKP LVDKENIIER LDAVEELAFN SNSQVQAIRK 

       490        500        510        520        530        540 
MLYRLPDLEK GLSRIYYQRG FYKAASAFSK NSYSCFKSAL LRRLIQQLPS ISSIIDHFLG 

       550        560        570        580        590        600 
MFDQKEAENN NKVDMFKDID NFDLSEEPND VDYELAQEIR ELKMSILMVR TEMDFHLQEL 

       610        620        630        640        650        660 
RDYLEYPNLE FSIWGNVKFC IEVSKGCKKI PPDWIKLSST RSLFRFHTPK IQSLLIELSS 

       670        680        690        700        710        720 
HEENLTISSE KIYRSFLSRI SEHYNELRNV TTVLGTLDCL ISFARISSQS GYTRPEFSDK 

       730        740        750        760        770        780 
ELLIHESRHP MIELLSDKSF VPNHIHLSSD GVRCLLITGP NMGGKSSFVK QLALSAIMAQ 

       790        800        810        820        830        840 
SGCFVPAKSA LLPIFDSILI RMGSSDNLSV NMSTFMVEML ETKEVLSKAT EKSMVIIDEL 

       850        860        870        880        890        900 
GRGTSTIDGE AISYAVLHYL NQYIKSYLLF VTHFPSLGIL ERRFEGQLRC FHMGYLKSKE 

       910        920        930        940        950        960 
DFETSVSQSI SFLYKLVPGV ASKSYGLNVA RMAGIPFSIL SRATEISENY EKKHRNARKN 

       970        980        990 
VFIRKVAKLL MILNAEEIDF KRLFYDLTAF EEI 

References and documentation are available.

Number of amino acids: 993

Molecular weight: 113362.04

Theoretical pI: 7.12

Amino acid composition: 
Ala (A)  50	  5.0%
Arg (R)  54	  5.4%
Asn (N)  43	  4.3%
Asp (D)  47	  4.7%
Cys (C)  14	  1.4%
Gln (Q)  41	  4.1%
Glu (E)  73	  7.4%
Gly (G)  41	  4.1%
His (H)  25	  2.5%
Ile (I)  70	  7.0%
Leu (L) 113	 11.4%
Lys (K)  65	  6.5%
Met (M)  26	  2.6%
Phe (F)  56	  5.6%
Pro (P)  25	  2.5%
Ser (S) 113	 11.4%
Thr (T)  42	  4.2%
Trp (W)   4	  0.4%
Tyr (Y)  34	  3.4%
Val (V)  57	  5.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 120
Total number of positively charged residues (Arg + Lys): 119

Atomic composition:

Carbon      C	      5067
Hydrogen    H	      8024
Nitrogen    N	      1358
Oxygen      O	      1507
Sulfur      S	        40

Formula: C5067H8024N1358O1507S40
Total number of atoms: 15996

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    73535
Abs 0.1% (=1 g/l)   0.649, assuming all pairs of Cys residues form cystines


Ext. coefficient    72660
Abs 0.1% (=1 g/l)   0.641, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.04
This classifies the protein as unstable.



Aliphatic index: 93.55

Grand average of hydropathicity (GRAVY): -0.201