ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRICHSRTLS NLKDLPITSR RAMHSAIVNY STQKAQFPVE TNNGEHYWAE KPNKFYQNKR 

        70         80         90        100        110        120 
PNFQGITFAK QQDLPSLPVP ELKSTLDKYL QTIRPFCNDV ETFERQQLLC KDFSEHMGPI 

       130        140        150        160        170        180 
LQDRLKEYAN DKRNWMAKFW DEQSYLQYND PIVPYVSYFY SHMPLPNHLS KIDNDPLIKA 

       190        200        210        220        230        240 
TAIISTVVKF IEAIKDESLP VEIIKGMPFC MNSFSLMFNT SRLPGKPEDN QDTNIFYSVY 

       250        260        270        280        290        300 
ENNFVTIAYK GKFYKLMTHD GNDKPLSENE IWRQLYSVVF QGSQSDPKLG GIGSLTSLPR 

       310        320        330        340        350        360 
DQWREVHLEL MKDPISQDSL ETIHKSSFML CLDLDQSPVT LEEKSRNCWH GDGINRFYDK 

       370        380        390        400        410        420 
SLQFLVTGNG SSGFLAEHSK MDGTPTLFLN NYVCQQLNKL DVDDFMRKVI TPSSTVAMKP 

       430        440        450        460        470        480 
MELPFIITPK IHKAIESAQL QFKETIGEHD LRVWHYNKYG KTFIKRHGMS PDAFIQQVIQ 

       490        500        510        520        530        540 
LAVFKYLKRQ LPTYEAASTR KYFKGRTETG RSVSTASLEF VSKWQNGDVP IAEKIQALKH 

       550        560        570        580        590        600 
SAKEHSTYLK NAANGNGVDR HFFGLKNMLK SNDDQIPPLF KDPLFNYSST WLISTSQLSS 

       610        620        630        640        650        660 
EYFDGYGWSQ VNDNGFGLAY MLNNEWLHIN IVNKPAKSGA SVNRLHYYLS QAADEIFDAL 

       670 
ENENKRKAKL 

References and documentation are available.

Number of amino acids: 670

Molecular weight: 77241.79

Theoretical pI: 8.35

Amino acid composition: 
Ala (A)  32	  4.8%
Arg (R)  25	  3.7%
Asn (N)  44	  6.6%
Asp (D)  39	  5.8%
Cys (C)   7	  1.0%
Gln (Q)  34	  5.1%
Glu (E)  36	  5.4%
Gly (G)  31	  4.6%
His (H)  20	  3.0%
Ile (I)  38	  5.7%
Leu (L)  61	  9.1%
Lys (K)  54	  8.1%
Met (M)  18	  2.7%
Phe (F)  37	  5.5%
Pro (P)  35	  5.2%
Ser (S)  56	  8.4%
Thr (T)  33	  4.9%
Trp (W)  11	  1.6%
Tyr (Y)  29	  4.3%
Val (V)  30	  4.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 79

Atomic composition:

Carbon      C	      3479
Hydrogen    H	      5342
Nitrogen    N	       928
Oxygen      O	      1017
Sulfur      S	        25

Formula: C3479H5342N928O1017S25
Total number of atoms: 10791

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   104085
Abs 0.1% (=1 g/l)   1.348, assuming all pairs of Cys residues form cystines


Ext. coefficient   103710
Abs 0.1% (=1 g/l)   1.343, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.27
This classifies the protein as unstable.



Aliphatic index: 75.39

Grand average of hydropathicity (GRAVY): -0.544