ProtParam
User-provided sequence:
10 20 30 40 50 60
MRIFPNLINL NSLTETKLLE LSSHFVAGNH VEFPSDRKFE VIEPRSGKPM ATWHYATRDQ
70 80 90 100 110 120
VDLTVKEAKK AQKQWAKSSW MERSEILKKT GDLLKTHCND IAYWECISNG KPIAEAKADV
130 140 150 160 170 180
LSCVDTFYFY SGIASDLLGQ HVPLDASRYA YTRRLPVGVV AAIGAWNYPI QTCSWKTAPA
190 200 210 220 230 240
LACGNAVIYK PSPLSPVTAL ILAEILKSAG LPDGVFNVIQ GDAETAQDLI HHDGVSKVSF
250 260 270 280 290 300
TGSIPTGKKI MKACADRNIK PVTLELGGKS ALIVFDDADI DSAVSCAMMA NFYSQGQVCS
310 320 330 340 350 360
NASKVLVHKS VLKEFTKKLV DHTQKMKIGD PLKEDTQVGS HISAEHRNKV EGYISTAIAE
370 380 390 400 410 420
GATKLCGGDR VAVHGLENGF YLSPCILTGI TPKMTVYREE IFGSVLLIIP FDTEDEAIKI
430 440 450 460 470 480
ANDTDMGLAA GLVTKDLSRS YRVSEQLNAG NVYVNTYNDV SPLVPFGGVG ESGFGRENGT
490 500
AVLEHYTHLK SVFVNTGTCP NPF
References and
documentation are available.
Number of amino acids: 503
Molecular weight: 54742.59
Theoretical pI: 6.41
Total number of negatively charged residues (Asp + Glu): 55
Total number of positively charged residues (Arg + Lys): 51
Atomic composition:
Carbon C 2443
Hydrogen H 3852
Nitrogen N 652
Oxygen O 734
Sulfur S 20
Formula: C
2443H
3852N
652O
734S
20
Total number of atoms: 7701
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 57465
Abs 0.1% (=1 g/l) 1.050, assuming all pairs of Cys residues form cystines
Ext. coefficient 56840
Abs 0.1% (=1 g/l) 1.038, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.15
This classifies the protein as stable.
Aliphatic index: 88.99
Grand average of hydropathicity (GRAVY): -0.099