ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRIGMEELRR ALALLNGQGY EFMVFIKEKD HLISESIPKN LFSESFISSS IEKNPPLTFD 

        70         80         90        100        110        120 
PLVNRKRITV YEENIQPAIM LPLDQPLLIQ NYLYTAFKLL RQVPCKAIAK LWIKIIEPRK 

       130        140        150        160        170        180 
KTRFPYIKGN AGRPEWWPKD VEHKEPDHLQ KADRLKLMCT IIMNVLPQSP FSLEILDELT 

       190        200        210        220        230        240 
GVTAAMTIFK KETVKKVIMK NVFEIAKCLC NKDFKQKTIS LDDLNDLAQK QKKSYHSRRF 

       250        260        270        280        290        300 
DVNKMVKVEK DLLQQSSGSD SSPSMILPSK ESDTEYFPRE YPKDPVSEDY SNSISRLENL 

       310        320 
VEFDPLFLTK LDELTSSDDC KDFI 

References and documentation are available.

Number of amino acids: 324

Molecular weight: 37603.70

Theoretical pI: 8.10

Amino acid composition: 
Ala (A)  12	  3.7%
Arg (R)  14	  4.3%
Asn (N)  14	  4.3%
Asp (D)  22	  6.8%
Cys (C)   5	  1.5%
Gln (Q)  12	  3.7%
Glu (E)  25	  7.7%
Gly (G)   7	  2.2%
His (H)   4	  1.2%
Ile (I)  25	  7.7%
Leu (L)  36	 11.1%
Lys (K)  35	 10.8%
Met (M)  10	  3.1%
Phe (F)  16	  4.9%
Pro (P)  21	  6.5%
Ser (S)  26	  8.0%
Thr (T)  13	  4.0%
Trp (W)   3	  0.9%
Tyr (Y)   9	  2.8%
Val (V)  15	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      1696
Hydrogen    H	      2708
Nitrogen    N	       438
Oxygen      O	       493
Sulfur      S	        15

Formula: C1696H2708N438O493S15
Total number of atoms: 5350

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30160
Abs 0.1% (=1 g/l)   0.802, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   0.795, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.84
This classifies the protein as unstable.



Aliphatic index: 90.56

Grand average of hydropathicity (GRAVY): -0.427