ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRIISSIFAP TRGFHSRSTW ILLPTTSSTA CGANQLRMSS EKAVPCYEES VRLLNGLQSN 

        70         80         90        100        110        120 
AATIKKLRVQ RENLQAINLP QCRKYLESLN ISAEDLNALN IIHVSGTKGK GSACAFVESI 

       130        140        150        160        170        180 
LRSQGLRTGF YSSPHLVHVR ERIQVDGQPV SEQMFAEEFF HVYDIIKREH SDNMPAYFKF 

       190        200        210        220        230        240 
LTLLAFRIFV KLNVQVMILE VGIGGEYDCT NVVEKPKVCG VTTLDYDHMS ILGNKLSEIA 

       250        260        270        280        290        300 
WHKAGIFKES VPAFYSPTTT EAEEVLIARA ISKHVPLFQT PPVSAYQFAR DISPGIRGAH 

       310        320        330        340        350        360 
QFSNVSMALQ LVRAWAEKCG FPLPGVPLST DTSGFNVPLW MCDAIESCRW PGRSQIVSTD 

       370        380        390        400        410        420 
RNVTYLLDGA HTPKSMEACS EWAAEEIVNL KKENVKKILL FQCTADRCPS TLIKYLKPLG 

       430        440        450        460        470        480 
ISQIVSCPTQ LHSSIDKSAD SANLNASRDE QAEKANQCVQ AWKESLDQPE SVTEDQMKVF 

       490        500        510        520 
DCISSAYKFI ESQAASQEIL VLVTGSLHLV GGVLNLAGKG K 

References and documentation are available.

Number of amino acids: 521

Molecular weight: 57655.16

Theoretical pI: 7.17

Amino acid composition: 
Ala (A)  42	  8.1%
Arg (R)  23	  4.4%
Asn (N)  21	  4.0%
Asp (D)  19	  3.6%
Cys (C)  15	  2.9%
Gln (Q)  25	  4.8%
Glu (E)  34	  6.5%
Gly (G)  28	  5.4%
His (H)  13	  2.5%
Ile (I)  34	  6.5%
Leu (L)  48	  9.2%
Lys (K)  30	  5.8%
Met (M)  10	  1.9%
Phe (F)  21	  4.0%
Pro (P)  24	  4.6%
Ser (S)  51	  9.8%
Thr (T)  26	  5.0%
Trp (W)   7	  1.3%
Tyr (Y)  12	  2.3%
Val (V)  38	  7.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2561
Hydrogen    H	      4063
Nitrogen    N	       699
Oxygen      O	       763
Sulfur      S	        25

Formula: C2561H4063N699O763S25
Total number of atoms: 8111

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    57255
Abs 0.1% (=1 g/l)   0.993, assuming all pairs of Cys residues form cystines


Ext. coefficient    56380
Abs 0.1% (=1 g/l)   0.978, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.36
This classifies the protein as unstable.



Aliphatic index: 90.60

Grand average of hydropathicity (GRAVY): -0.102