ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRIKKKNTRG NARNFITRSQ AVRKLQVSLA DFRRLCIFKG IYPREPRNKK KANKGSTAPT 

        70         80         90        100        110        120 
TFYYAKDIQY LMHEPVLAKF REHKTFARKL TRALGRGEVS SAKRLEENRD SYTLDHIIKE 

       130        140        150        160        170        180 
RYPSFPDAIR DIDDALNMLF LFSNLPSTNQ VSSKIINDAQ KICNQWLAYV AKERLVRKVF 

       190        200        210        220        230        240 
VSIKGVYYQA NIKGEEVRWL VPFKFPENIP SDVDFRIMLT FLEFYSTLLH FVLYKLYTDS 

       250        260        270        280        290        300 
GLIYPPKLDL KKDKIISGLS SYILESRQED SLLKLDPTEI EEDVKVESLD ASTLKSALNA 

       310        320        330        340        350        360 
DEANTDETEK EEEQEKKQEK EQEKEQNEET ELDTFEDNNK NKGDILIQPS KYDSPVASLF 

       370        380        390        400        410        420 
SAFVFYVSRE VPIDILEFLI LSCGGNVISE AAMDQIENKK DIDMSKVTHQ IVDRPVLKNK 

       430        440        450        460        470        480 
VAGRTYIQPQ WIFDCINKGE LVPANKYLPG EALPPHLSPW GDAIGYDPTA PVEEGEEEES 

       490        500        510        520        530        540 
ESESESEDQV EEEDQEVVAG EEDDDDDEEL QAQKELELEA QGIKYSETSE ADKDVNKSKN 

       550        560        570        580        590        600 
KKRKVDEEEE EKKLKMIMMS NKQKKLYKKM KYSNAKKEEQ AENLKKKKKQ IAKQKAKLNK 


LDSKK 

References and documentation are available.

Number of amino acids: 605

Molecular weight: 69877.19

Theoretical pI: 5.48

Amino acid composition: 
Ala (A)  37	  6.1%
Arg (R)  26	  4.3%
Asn (N)  30	  5.0%
Asp (D)  43	  7.1%
Cys (C)   4	  0.7%
Gln (Q)  26	  4.3%
Glu (E)  70	 11.6%
Gly (G)  20	  3.3%
His (H)   6	  1.0%
Ile (I)  37	  6.1%
Leu (L)  52	  8.6%
Lys (K)  75	 12.4%
Met (M)  10	  1.7%
Phe (F)  22	  3.6%
Pro (P)  25	  4.1%
Ser (S)  42	  6.9%
Thr (T)  22	  3.6%
Trp (W)   4	  0.7%
Tyr (Y)  22	  3.6%
Val (V)  32	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 113
Total number of positively charged residues (Arg + Lys): 101

Atomic composition:

Carbon      C	      3100
Hydrogen    H	      4946
Nitrogen    N	       830
Oxygen      O	       974
Sulfur      S	        14

Formula: C3100H4946N830O974S14
Total number of atoms: 9864

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55030
Abs 0.1% (=1 g/l)   0.788, assuming all pairs of Cys residues form cystines


Ext. coefficient    54780
Abs 0.1% (=1 g/l)   0.784, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 58.52
This classifies the protein as unstable.



Aliphatic index: 78.83

Grand average of hydropathicity (GRAVY): -0.816