ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRIQLNTIDL QCIIALSCLG QFVHAEANRE DLKQIDFQFP VLERAATKTP FPDWLSAFTG 

        70         80         90        100        110        120 
LKEWPGLDPP YIPLDFIDFS QIPDYKEYDQ NHCDSVPRDS CSFDCHHCTE HDDVYTCSKL 

       130        140        150        160        170        180 
SQTFDDGPSA STTKLLDRLK HNSTFFNLGV NIVQHPDIYQ RMQKEGHLIG SHTWSHVYLP 

       190        200        210        220        230        240 
NVSNEKIIAQ IEWSIWAMNA TGNHTPKWFR PPYGGIDNRV RAITRQFGLQ AVLWDHDTFD 

       250        260        270        280        290        300 
WSLLLNDSVI TEQEILQNVI NWNKSGTGLI LEHDSTEKTV DLAIKINKLI GDDQSTVSHC 

       310 
VGGIDYIKEF LS 

References and documentation are available.

Number of amino acids: 312

Molecular weight: 35693.15

Theoretical pI: 5.12

Amino acid composition: 
Ala (A)  13	  4.2%
Arg (R)  10	  3.2%
Asn (N)  16	  5.1%
Asp (D)  28	  9.0%
Cys (C)   8	  2.6%
Gln (Q)  17	  5.4%
Glu (E)  14	  4.5%
Gly (G)  16	  5.1%
His (H)  14	  4.5%
Ile (I)  26	  8.3%
Leu (L)  28	  9.0%
Lys (K)  15	  4.8%
Met (M)   3	  1.0%
Phe (F)  15	  4.8%
Pro (P)  15	  4.8%
Ser (S)  22	  7.1%
Thr (T)  20	  6.4%
Trp (W)   9	  2.9%
Tyr (Y)   8	  2.6%
Val (V)  15	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 25

Atomic composition:

Carbon      C	      1601
Hydrogen    H	      2431
Nitrogen    N	       427
Oxygen      O	       480
Sulfur      S	        11

Formula: C1601H2431N427O480S11
Total number of atoms: 4950

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    61920
Abs 0.1% (=1 g/l)   1.735, assuming all pairs of Cys residues form cystines


Ext. coefficient    61420
Abs 0.1% (=1 g/l)   1.721, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.74
This classifies the protein as stable.



Aliphatic index: 85.61

Grand average of hydropathicity (GRAVY): -0.365