ProtParam
User-provided sequence:
10 20 30 40 50 60
MRIQSLFVLF NVAIIAWSYP YEPLRVLQVG ENEVMEVPES EKLNLRRRGV KFFDVTKHTS
70 80 90 100 110 120
FLPFFNKEEE PTVPTYNYPP EISNKEVVDD SIKNIDKGSM HKNLAKFTSF YTRYYKSDHG
130 140 150 160 170 180
FESAEWLAAT IANITKDIPQ DTLTIEHFDH KEWKQYSIIV RVTGSTTPED IIIIGSHQDS
190 200 210 220 230 240
INLLLPSIMA APGADDNGSG TVTNMEALRL YTENFLKRGF RPNNTVEFHF YSAEEGGLLG
250 260 270 280 290 300
SLDVFTAYAK QKKHVRAMLQ QDMTGYVSDP EDEHVGIVTD YTTPALTDFI KLIINSYLSI
310 320 330 340 350 360
PYRDTQCGYA CSDHGSATRN GFPGSFVIES EFKKTNKYIH STMDTLDRLS LAHMAEHTKI
370
VLGVIIELGS WSAW
References and
documentation are available.
Number of amino acids: 374
Molecular weight: 42484.04
Theoretical pI: 5.48
Total number of negatively charged residues (Asp + Glu): 49
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 1917
Hydrogen H 2936
Nitrogen N 496
Oxygen O 575
Sulfur S 11
Formula: C
1917H
2936N
496O
575S
11
Total number of atoms: 5935
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 52955
Abs 0.1% (=1 g/l) 1.246, assuming all pairs of Cys residues form cystines
Ext. coefficient 52830
Abs 0.1% (=1 g/l) 1.244, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.45
This classifies the protein as unstable.
Aliphatic index: 81.84
Grand average of hydropathicity (GRAVY): -0.313