ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRIYQCHFCS SPCYPGHGIM FVRNDAKEFR FCRSKCHKAF KQRRNPRKLK WTKAFRKAAG 

        70         80         90        100        110        120 
KELAVDSTLT FAQRRNVPVR YNRELVATTL KAMARIEEIR QKRERAFYKN RMRGNKEKDF 

       130        140        150        160        170        180 
LRDKKLVESN PELLRIREVE IARKLAKEQE RAESVSEQEE SEEEEEDMEI DSDEEEEEQL 

       190 
EKQKILLKNR RRNTKKIAF 

References and documentation are available.

Number of amino acids: 199

Molecular weight: 23975.48

Theoretical pI: 9.61

Amino acid composition: 
Ala (A)  15	  7.5%
Arg (R)  26	 13.1%
Asn (N)   9	  4.5%
Asp (D)   7	  3.5%
Cys (C)   5	  2.5%
Gln (Q)   8	  4.0%
Glu (E)  29	 14.6%
Gly (G)   4	  2.0%
His (H)   3	  1.5%
Ile (I)   9	  4.5%
Leu (L)  13	  6.5%
Lys (K)  23	 11.6%
Met (M)   5	  2.5%
Phe (F)  10	  5.0%
Pro (P)   5	  2.5%
Ser (S)   9	  4.5%
Thr (T)   6	  3.0%
Trp (W)   1	  0.5%
Tyr (Y)   4	  2.0%
Val (V)   8	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 49

Atomic composition:

Carbon      C	      1039
Hydrogen    H	      1696
Nitrogen    N	       324
Oxygen      O	       308
Sulfur      S	        10

Formula: C1039H1696N324O308S10
Total number of atoms: 3377

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    11710
Abs 0.1% (=1 g/l)   0.488, assuming all pairs of Cys residues form cystines


Ext. coefficient    11460
Abs 0.1% (=1 g/l)   0.478, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 71.03
This classifies the protein as unstable.



Aliphatic index: 62.31

Grand average of hydropathicity (GRAVY): -1.148