ProtParam
User-provided sequence:
10 20 30 40 50 60
MRIYYIGVFR SGGEKALELS EVKDLSQFGF FERSSVGQFM TFFAETVASR TGAGQRQSIE
70 80 90 100 110 120
EGNYIGHVYA RSEGICGVLI TDKEYPVRPA YTLLNKILDE YLVAHPKEEW ADVTETNDAL
130 140 150 160 170 180
KMKQLDTYIS KYQDPSQADA IMKVQQELDE TKIVLHKTIE NVLQRGEKLD NLVDKSESLT
190 200
ASSKMFYKQA KKSNSCCIIM
References and
documentation are available.
Number of amino acids: 200
Molecular weight: 22706.84
Theoretical pI: 5.52
Total number of negatively charged residues (Asp + Glu): 29
Total number of positively charged residues (Arg + Lys): 25
Atomic composition:
Carbon C 1007
Hydrogen H 1591
Nitrogen N 265
Oxygen O 313
Sulfur S 9
Formula: C
1007H
1591N
265O
313S
9
Total number of atoms: 3185
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20525
Abs 0.1% (=1 g/l) 0.904, assuming all pairs of Cys residues form cystines
Ext. coefficient 20400
Abs 0.1% (=1 g/l) 0.898, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.71
This classifies the protein as unstable.
Aliphatic index: 81.90
Grand average of hydropathicity (GRAVY): -0.400