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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRIYYIGVFR SGGEKALELS EVKDLSQFGF FERSSVGQFM TFFAETVASR TGAGQRQSIE

70 80 90 100 110 120
EGNYIGHVYA RSEGICGVLI TDKEYPVRPA YTLLNKILDE YLVAHPKEEW ADVTETNDAL

130 140 150 160 170 180
KMKQLDTYIS KYQDPSQADA IMKVQQELDE TKIVLHKTIE NVLQRGEKLD NLVDKSESLT

190 200
ASSKMFYKQA KKSNSCCIIM


References and documentation are available.

Number of amino acids: 200 Molecular weight: 22706.84 Theoretical pI: 5.52
Amino acid composition: 
Ala (A) 13 6.5% Arg (R) 8 4.0% Asn (N) 6 3.0% Asp (D) 11 5.5% Cys (C) 3 1.5% Gln (Q) 11 5.5% Glu (E) 18 9.0% Gly (G) 12 6.0% His (H) 3 1.5% Ile (I) 13 6.5% Leu (L) 16 8.0% Lys (K) 17 8.5% Met (M) 6 3.0% Phe (F) 8 4.0% Pro (P) 4 2.0% Ser (S) 16 8.0% Thr (T) 11 5.5% Trp (W) 1 0.5% Tyr (Y) 10 5.0% Val (V) 13 6.5% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 29 Total number of positively charged residues (Arg + Lys): 25 Atomic composition: Carbon C 1007 Hydrogen H 1591 Nitrogen N 265 Oxygen O 313 Sulfur S 9 Formula: C1007H1591N265O313S9 Total number of atoms: 3185 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 20525 Abs 0.1% (=1 g/l) 0.904, assuming all pairs of Cys residues form cystines Ext. coefficient 20400 Abs 0.1% (=1 g/l) 0.898, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 46.71 This classifies the protein as unstable. Aliphatic index: 81.90 Grand average of hydropathicity (GRAVY): -0.400