ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRKDADMAIF GEAAPYLRKS EKERIEAQNK PFDAKSSVFV ADPKESFVKA TVQSREGGKV 

        70         80         90        100        110        120 
TAKTEAGATV TVKEDQVFPM NPPKYDKIED MAMMTHLHEP AVLYNLKEXY AAWMIYTYSG 

       130        140        150        160        170        180 
LFCVTVNPYK WLPVYNAEVV TAYRGKKRQE APPHIFSISD NAYQFMLTDR ENQSILITGE 

       190        200        210        220        230        240 
SGAGKTVNTK RVIQYFATIA VTGDKKKEEA TSGKMQGTLE DQIISANPLL EAFANAKTVR 

       250        260        270        280        290        300 
NDNSSRFGKF IRIHFGTTGK LASADIETYL LEKSRVTFQL KAERSYHIFY QIMSNKKPDL 

       310        320        330        340        350        360 
IEMLLITTNP YDYAFVSQGE ITVPSIDDQE ELMATDSAID ILGFTSDERV SIYKLTGAVM 

       370        380        390        400        410        420 
HYGNMKFKQK QREEQAEPDG TEVADKAAYL QSLNSXDLLK ALCYPRVKVG NEYVTKGQTV 

       430        440        450        460        470        480 
QQVYNAVGAL AKAVYEKMFL WMVTRINQQL DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC 

       490        500        510        520        530        540 
INFTNEKLQQ FFNHHMFVLE QEEYKKEGIE WEFIDFGMDL AACIELIEKP MGIFSILEEE 

       550        560        570        580        590        600 
CMFPKATDTS FKNKLYEQHL GKSNNFQKPK PAKRKVEAHF SLVHYAGTVD YNITGWLDKN 

       610        620        630        640        650        660 
KDPLNETVVG LYQKSAMKTL AFLFTGTAAA EAEGGGKKGG KKKGSSFQTV SALFRENLNK 

       670        680        690        700        710        720 
LMTNLRTHSP HFVRSIIPNE TKTPGAMEHE LVLHQLRCNG VLEGIRICRK GFPSRILYAD 

       730        740        750        760        770        780 
FKQRYKVLNA SAIPEGQFID SKKASEKLLG SIDVDHTQYK FGHTKVFFKA GLLGLLEEMR 

       790        800        810        820        830        840 
DDKLAQLITR TQAMCRGFLA RVEYKKMVER RESIFCIQYN IRAFMNVKHW PWMKLYFKIK 

       850        860        870        880        890        900 
PLLKSAETEK EMANMKEEFE KTKESLAKAE AKEKELEEKM VALMQEKNDL QLQVQAEADS 

       910        920        930        940        950        960 
LADAEERCDQ LIKTKIQLEA KIKEVTERAE DEEEINAELT AKKRKLEDEC SELKKDIDDL 

       970        980        990       1000       1010       1020 
ELTLAKVEKE KHATENKVKN LTEEMAGLDE TIAKLTKEKK ALQEAHQQTL DDLQAEEDKV 

      1030       1040       1050       1060       1070       1080 
NTLTKAKTKL EQQVDDLEGS LEQEKKIRMD LERAKRKLEG DLKLAQESTM DIENDKQQLD 

      1090       1100       1110       1120       1130       1140 
EKLKKKEFEM SNLQSKIEDE QALAMQLQKK IKELQARIEE LEEEIEAERA SRAKAEKQRS 

      1150       1160       1170       1180       1190       1200 
DLSRELEEIS ERLEEAGGAT SAQIEMNKKR EAEFQKMRRD LEEATLQHEA TAATLRKKHA 

      1210       1220       1230       1240       1250       1260 
DSVAELGEQI DNLQRVKQKL EKEKSELKME IDDLASNMET VSKAKGNLEK MCRTLEDQVS 

      1270       1280       1290       1300       1310       1320 
ELKTKEEEHQ RLINDLSAQR ARLQTESGEF SRQLDEKDSL VSQLSRGKQA FTQQIEELKR 

      1330       1340       1350       1360       1370       1380 
QLEEEIKAKS ALAHALQSAR HDCDLLREQY EEEQEAKAEL QRAMSKANSE VAQWRTKYET 

      1390       1400       1410       1420       1430       1440 
DAIQRTEELE EAKKKLAQRL QDAEEHVEAV NAKCASLEKT KQRLQNEVED LMIDVERTNA 

      1450       1460       1470       1480       1490       1500 
ACAALDKKQR NFDKILAEWK HKYEETHAEL EASQKESRSL STEVFKVKNA YEESLDQLET 

      1510       1520       1530       1540       1550       1560 
LKRENKNLQQ EISDLTEQIA EGGKRIHELE KVKKQVEQEK SELQAALEEA EASLEHEEGK 

      1570       1580       1590       1600       1610       1620 
ILRIQLELNQ VKSEIDRKIA EKDEEIDQLK RNHIRVVESM QSTLDAEIRS RNDAIRIKKK 

      1630       1640       1650       1660       1670       1680 
MEGDLNEMEI QLNHANRMAA EALRNYRNTQ GILKDTQLHL DDALRGQEDL KEQLAMVERR 

      1690       1700       1710       1720       1730       1740 
ANLLQAEIEE LRATLEQTER SRKVAEQELL DASERVQLLH TQNTSLINTK KKLETDISQI 

      1750       1760       1770       1780       1790       1800 
QGEMEDIVQE ARNAEEKAKK AITDAAMMAE ELKKEQDTSA HLERMKKNME QTVKDLQHRL 

      1810       1820       1830       1840       1850       1860 
DEAEQLALKG GKKQIQKLEA RVRELEAEVE SEQKRNVEAV KGLRKHERRV KELTYQTEED 

      1870       1880       1890       1900       1910       1920 
RKNVLRLQDL VDKLQAKVKS YKRQAEEAEE QCNINLSKFR KLQHELEEAE ERADIAESQV 

      1930 
NKLRVKSREV HTKVISEE 

References and documentation are available.

Number of amino acids: 1938

Molecular weight: 223067.62

Theoretical pI: 5.62

Amino acid composition: 
Ala (A) 176	  9.1%
Arg (R) 103	  5.3%
Asn (N)  76	  3.9%
Asp (D) 100	  5.2%
Cys (C)  16	  0.8%
Gln (Q) 127	  6.6%
Glu (E) 260	 13.4%
Gly (G)  68	  3.5%
His (H)  39	  2.0%
Ile (I)  95	  4.9%
Leu (L) 190	  9.8%
Lys (K) 211	 10.9%
Met (M)  54	  2.8%
Phe (F)  57	  2.9%
Pro (P)  30	  1.5%
Ser (S)  94	  4.9%
Thr (T)  99	  5.1%
Trp (W)   9	  0.5%
Tyr (Y)  42	  2.2%
Val (V)  90	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   2	  0.1%


Total number of negatively charged residues (Asp + Glu): 360
Total number of positively charged residues (Arg + Lys): 314


Atom composition:

As there is at least one ambiguous position (B,Z or X) in the sequence
considered, the atomic composition cannot be computed.

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   113080
Abs 0.1% (=1 g/l)   0.507, assuming all pairs of Cys residues form cystines


Ext. coefficient   112080
Abs 0.1% (=1 g/l)   0.502, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.92
This classifies the protein as unstable.



Aliphatic index: 79.90

Grand average of hydropathicity (GRAVY): -0.783