ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRKLTAVNSL LKRNNYLLPR HGSSQTAAQR TSSVRETETE TKLPKMPPFN YSPPPYDGPS 

        70         80         90        100        110        120 
TAEIIAKRRE FLSPALFHFY NTPLNIVEAK MQYVFDENGR RYLDAFGGIA TVSCGHCHPE 

       130        140        150        160        170        180 
VVNSVVKQLK LINHSTILYL NHTISDFAEA LVSTLPGDLK VVFFTNSGTE ANELAMMMAR 

       190        200        210        220        230        240 
LYTGCNDIVS LRNSYHGNAA ATMGATAQSN WKFNVVQSGV HHAINPDPYR GIFGSDGEKY 

       250        260        270        280        290        300 
ASDVHDLIQF GTSGQVAGFI GESIQGVGGI VELAPGYLPA AYDIVRKAGG VCIADEVQSG 

       310        320        330        340        350        360 
FARTGTHFWG FQSHGVIPDI VTMAKGIGNG IPLGAVVTTP EIAGVLSRRS YFNTFGGNPM 

       370        380        390        400        410        420 
CTAAGHAVLR VLHEEKLQEN ANLVGSHLKR RLTLLKNKYE LIGDVRGRGL MLGVEFVKDR 

       430        440        450        460        470        480 
DLKTPAKAET LHLMDQMKEM GVLVGKGGFY GNVFRITPPL CFTLSDADFL VDVMDHAMSK 


M 

References and documentation are available.

Number of amino acids: 481

Molecular weight: 52461.07

Theoretical pI: 8.12

Amino acid composition: 
Ala (A)  39	  8.1%
Arg (R)  23	  4.8%
Asn (N)  25	  5.2%
Asp (D)  21	  4.4%
Cys (C)   6	  1.2%
Gln (Q)  13	  2.7%
Glu (E)  23	  4.8%
Gly (G)  47	  9.8%
His (H)  17	  3.5%
Ile (I)  23	  4.8%
Leu (L)  43	  8.9%
Lys (K)  23	  4.8%
Met (M)  16	  3.3%
Phe (F)  23	  4.8%
Pro (P)  23	  4.8%
Ser (S)  29	  6.0%
Thr (T)  30	  6.2%
Trp (W)   2	  0.4%
Tyr (Y)  16	  3.3%
Val (V)  39	  8.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 44
Total number of positively charged residues (Arg + Lys): 46

Atomic composition:

Carbon      C	      2337
Hydrogen    H	      3667
Nitrogen    N	       647
Oxygen      O	       683
Sulfur      S	        22

Formula: C2337H3667N647O683S22
Total number of atoms: 7356

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    35215
Abs 0.1% (=1 g/l)   0.671, assuming all pairs of Cys residues form cystines


Ext. coefficient    34840
Abs 0.1% (=1 g/l)   0.664, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.42
This classifies the protein as stable.



Aliphatic index: 85.14

Grand average of hydropathicity (GRAVY): -0.096