ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRKPSITITT AKAIITPDYT LIKSHSKYQL PSRFQKLDAD SPERSTVVKL FYRRFMRLKP 

        70         80         90        100        110        120 
FISNVKMVKD TYRDYVRYKF MKENYELKRY LVFNPDGLRS KINLELLSNT KCCERILPVT 

       130        140        150        160        170        180 
EMQRTLEFVL KSCSYLPETK VQKWDIARDN TYCRQILKNL LTMQYEKYRS ILHRGIGHDE 

       190        200        210        220        230 
LDVKFSHLKT TSSPLTKLNK TEKKKIPLFK VFSDFDTTLI YLNETLGTRL 

References and documentation are available.

Number of amino acids: 230

Molecular weight: 27378.09

Theoretical pI: 9.80

Amino acid composition: 
Ala (A)   4	  1.7%
Arg (R)  17	  7.4%
Asn (N)   9	  3.9%
Asp (D)  12	  5.2%
Cys (C)   4	  1.7%
Gln (Q)   6	  2.6%
Glu (E)  12	  5.2%
Gly (G)   4	  1.7%
His (H)   4	  1.7%
Ile (I)  14	  6.1%
Leu (L)  28	 12.2%
Lys (K)  27	 11.7%
Met (M)   6	  2.6%
Phe (F)  11	  4.8%
Pro (P)  10	  4.3%
Ser (S)  16	  7.0%
Thr (T)  21	  9.1%
Trp (W)   1	  0.4%
Tyr (Y)  13	  5.7%
Val (V)  11	  4.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 24
Total number of positively charged residues (Arg + Lys): 44

Atomic composition:

Carbon      C	      1240
Hydrogen    H	      1994
Nitrogen    N	       332
Oxygen      O	       344
Sulfur      S	        10

Formula: C1240H1994N332O344S10
Total number of atoms: 3920

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    25120
Abs 0.1% (=1 g/l)   0.918, assuming all pairs of Cys residues form cystines


Ext. coefficient    24870
Abs 0.1% (=1 g/l)   0.908, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 27.72
This classifies the protein as stable.



Aliphatic index: 86.83

Grand average of hydropathicity (GRAVY): -0.517