ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRLGNAYAYC KPSQNVGLKL DLLRGLPGYV GHATSRINRL ENQDNYSIKM MRSWPNAYGS 

        70         80         90        100        110        120 
ALNCSVFDGH GEKGAQLSQL LADKLCSSLD FPEPSWDKQD LKKLVQEYAR RFPEGNYWKH 

       130        140        150        160        170        180 
KLSTFEKFYN KFIKNCNSKQ ELLLMKEGDS AILGQNGGRM IFDKMGNIID KIALLTELDR 

       190        200        210        220        230        240 
LRLFYGFARF DLDQCCGLGT AAGSTASSIF LYPYDDPNAP IDEGKDDDSW IISHSGLLKL 

       250        260        270        280        290        300 
IVTQVGDSKI ILCDQDGIAH ALTTTHHINS SRERHRLSID PSRLDPDAFG ETRFLNNFAN 

       310        320        330        340        350        360 
TRSFGDVAGK PYGISSEPDI FSFLVGNTLH LPRSERSKLP FNGDECFLAL VTDGITNKLA 

       370        380        390        400        410        420 
DQEVVDLITS TVNSWGLKKA TPQFVAEETI KFIQAIATKH SDNATCVVVR LSNWGNWPNV 

       430        440 
DRTGPQRETK LMNAQSNETK LN 

References and documentation are available.

Number of amino acids: 442

Molecular weight: 49420.91

Theoretical pI: 6.57

Amino acid composition: 
Ala (A)  28	  6.3%
Arg (R)  22	  5.0%
Asn (N)  29	  6.6%
Asp (D)  33	  7.5%
Cys (C)   9	  2.0%
Gln (Q)  16	  3.6%
Glu (E)  20	  4.5%
Gly (G)  33	  7.5%
His (H)  10	  2.3%
Ile (I)  25	  5.7%
Leu (L)  48	 10.9%
Lys (K)  29	  6.6%
Met (M)   7	  1.6%
Phe (F)  21	  4.8%
Pro (P)  18	  4.1%
Ser (S)  35	  7.9%
Thr (T)  23	  5.2%
Trp (W)   7	  1.6%
Tyr (Y)  12	  2.7%
Val (V)  17	  3.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 51

Atomic composition:

Carbon      C	      2190
Hydrogen    H	      3423
Nitrogen    N	       609
Oxygen      O	       664
Sulfur      S	        16

Formula: C2190H3423N609O664S16
Total number of atoms: 6902

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    56880
Abs 0.1% (=1 g/l)   1.151, assuming all pairs of Cys residues form cystines


Ext. coefficient    56380
Abs 0.1% (=1 g/l)   1.141, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.63
This classifies the protein as stable.



Aliphatic index: 81.90

Grand average of hydropathicity (GRAVY): -0.416