ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRLISKVLVK TNCLEVGMRR APQWYSHYST TAGNARVNKK GSKVVPVLTG LALASIFAKK 

        70         80         90        100        110        120 
WYDDSQIKKA DATSVAVDAS ISAFPKKMGP PQWPFSTQYE LIGKGVRCVS SITFKAYGLG 

       130        140        150        160        170        180 
IYVAAEDKHL VSEVLDSKFL SQAFIDTAAP PSPENSHQDN LRAALNDPAK APILINNLLD 

       190        200        210        220        230        240 
SGIRLMSKNT PIKAGSFKLL MDGTKKSVLK NPDSQSQDKD RLEAGFQELH DCFRSVKGLV 

       250        260        270        280        290        300 
ARDDDFFIEL NKDCSMNLSY YARKKDEFVI LGTVKEPLIG KLLFAHYLAA VDPPSPEARK 

       310 
EVIDALVSLS 

References and documentation are available.

Number of amino acids: 310

Molecular weight: 34113.35

Theoretical pI: 9.14

Amino acid composition: 
Ala (A)  30	  9.7%
Arg (R)  12	  3.9%
Asn (N)  12	  3.9%
Asp (D)  22	  7.1%
Cys (C)   4	  1.3%
Gln (Q)   9	  2.9%
Glu (E)  12	  3.9%
Gly (G)  16	  5.2%
His (H)   5	  1.6%
Ile (I)  16	  5.2%
Leu (L)  32	 10.3%
Lys (K)  30	  9.7%
Met (M)   6	  1.9%
Phe (F)  13	  4.2%
Pro (P)  17	  5.5%
Ser (S)  29	  9.4%
Thr (T)  11	  3.5%
Trp (W)   3	  1.0%
Tyr (Y)   9	  2.9%
Val (V)  22	  7.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 42

Atomic composition:

Carbon      C	      1532
Hydrogen    H	      2446
Nitrogen    N	       410
Oxygen      O	       449
Sulfur      S	        10

Formula: C1532H2446N410O449S10
Total number of atoms: 4847

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    30160
Abs 0.1% (=1 g/l)   0.884, assuming all pairs of Cys residues form cystines


Ext. coefficient    29910
Abs 0.1% (=1 g/l)   0.877, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.89
This classifies the protein as stable.



Aliphatic index: 90.65

Grand average of hydropathicity (GRAVY): -0.195