ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRLLHHGEYG TKLIGGKCSI DGKLGHPCPL SRRRKKHLRE KEMGPQYVRM YGPKRKAIIR 

        70         80         90        100        110        120 
TGNPDDGINL PDTGRGTLTA ATIWSRAYHS NYSYLVRPKV VTLSKHRELM TTFLLYVLYV 


CIYISAFIK 

References and documentation are available.

Number of amino acids: 129

Molecular weight: 14792.41

Theoretical pI: 10.09

Amino acid composition: 
Ala (A)   5	  3.9%
Arg (R)  12	  9.3%
Asn (N)   3	  2.3%
Asp (D)   4	  3.1%
Cys (C)   3	  2.3%
Gln (Q)   1	  0.8%
Glu (E)   4	  3.1%
Gly (G)  12	  9.3%
His (H)   6	  4.7%
Ile (I)   9	  7.0%
Leu (L)  14	 10.9%
Lys (K)  11	  8.5%
Met (M)   4	  3.1%
Phe (F)   2	  1.6%
Pro (P)   7	  5.4%
Ser (S)   7	  5.4%
Thr (T)   9	  7.0%
Trp (W)   1	  0.8%
Tyr (Y)   9	  7.0%
Val (V)   6	  4.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 8
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	       665
Hydrogen    H	      1069
Nitrogen    N	       193
Oxygen      O	       175
Sulfur      S	         7

Formula: C665H1069N193O175S7
Total number of atoms: 2109

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    19035
Abs 0.1% (=1 g/l)   1.287, assuming all pairs of Cys residues form cystines


Ext. coefficient    18910
Abs 0.1% (=1 g/l)   1.278, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 19.81
This classifies the protein as stable.



Aliphatic index: 86.90

Grand average of hydropathicity (GRAVY): -0.388