ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRLRRFQSVE SGANNVVFIR TLGIEPEKLV HHILQDMYKT KKKKTRVILR MLPISGTCKA 

        70         80         90        100        110        120 
FLEDMKKYAE TFLEPWFKAP NKGTFQIVYK SRNNSHVNRE EVIRELAGIV CTLNSENKVD 

       130        140        150        160        170        180 
LTNPQYTVVV EIIKAVCCLS VVKDYMLFRK YNLQEVVKSP KDPSQLNSKQ GNGKEAKLES 

       190        200        210        220        230        240 
ADKSDQNNTA EGKNNQQVPE NTEELGQTKP TSNPQVVNEG GAKPELASQA TEGSKSNEND 


FS 

References and documentation are available.

Number of amino acids: 242

Molecular weight: 27310.07

Theoretical pI: 9.01

Amino acid composition: 
Ala (A)  12	  5.0%
Arg (R)  10	  4.1%
Asn (N)  21	  8.7%
Asp (D)   8	  3.3%
Cys (C)   4	  1.7%
Gln (Q)  13	  5.4%
Glu (E)  22	  9.1%
Gly (G)  12	  5.0%
His (H)   3	  1.2%
Ile (I)  10	  4.1%
Leu (L)  18	  7.4%
Lys (K)  26	 10.7%
Met (M)   5	  2.1%
Phe (F)   8	  3.3%
Pro (P)  11	  4.5%
Ser (S)  17	  7.0%
Thr (T)  14	  5.8%
Trp (W)   1	  0.4%
Tyr (Y)   6	  2.5%
Val (V)  21	  8.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 30
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      1194
Hydrogen    H	      1933
Nitrogen    N	       339
Oxygen      O	       374
Sulfur      S	         9

Formula: C1194H1933N339O374S9
Total number of atoms: 3849

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14690
Abs 0.1% (=1 g/l)   0.538, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.529, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.67
This classifies the protein as unstable.



Aliphatic index: 75.25

Grand average of hydropathicity (GRAVY): -0.700