ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRLTAQVINE APEILNPEGK LTLLLRDLQI TELENLAITQ NKYQVIDLSN NDLISLGNIP 

        70         80         90        100        110        120 
KRFNNLQCLL LSNNNISYID DESFPSDNHI TSITLFNNNI YQFQKSFKDK FPKLETLILL 

       130        140        150        160        170        180 
GNPITEMENY RYFIIWLIPS LKVLDFKKVK QAERKTSEDM FGTNRDEFNS LAQQMFKNEN 

       190        200        210        220        230 
TEIKLDGKSD RQVKNFVKKM TDEERQQLLK KLETATSIEE IERIENDLKE GAV 

References and documentation are available.

Number of amino acids: 233

Molecular weight: 27168.01

Theoretical pI: 5.11

Amino acid composition: 
Ala (A)   7	  3.0%
Arg (R)   9	  3.9%
Asn (N)  24	 10.3%
Asp (D)  14	  6.0%
Cys (C)   1	  0.4%
Gln (Q)  13	  5.6%
Glu (E)  22	  9.4%
Gly (G)   6	  2.6%
His (H)   1	  0.4%
Ile (I)  21	  9.0%
Leu (L)  30	 12.9%
Lys (K)  21	  9.0%
Met (M)   5	  2.1%
Phe (F)  12	  5.2%
Pro (P)   7	  3.0%
Ser (S)  13	  5.6%
Thr (T)  14	  6.0%
Trp (W)   1	  0.4%
Tyr (Y)   5	  2.1%
Val (V)   7	  3.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1209
Hydrogen    H	      1943
Nitrogen    N	       321
Oxygen      O	       375
Sulfur      S	         6

Formula: C1209H1943N321O375S6
Total number of atoms: 3854

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    12950
Abs 0.1% (=1 g/l)   0.477, assuming all pairs of Cys residues form cystines


Ext. coefficient    12950
Abs 0.1% (=1 g/l)   0.477, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.06
This classifies the protein as stable.



Aliphatic index: 97.08

Grand average of hydropathicity (GRAVY): -0.542