ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRMSLHLNKK LLKEKKTLQN YPSEYGFASD TDRNEKLIVV GYVLRIFAQR VTGKFVLMMI 

        70         80         90        100        110        120 
WDEVGEDKFE REKVLLDIEQ ECVEAYRRKV DHANVSRSRL HQELAESEAE LTHFLLCLGE 

       130        140        150        160        170        180 
RSVPGRPEKK GGTLREQLDS IAPALREMRL RKDERVKQFR SVKGEIQKIS AEIAGRSTYE 

       190        200        210        220        230        240 
DSTRKITIDD NDLSNKKLEE YQNELHRLHD EKNERLQKVD IYICAIRDLS ATLGTEASMI 

       250        260        270        280        290        300 
ITKIHPSLND LYGISKNISD DILKKLNGTV VSLEEEKHKR LEKLHHLGRA LSNLWNLMDA 

       310        320        330        340        350        360 
SYEDRQKFFH VIDLLSSAPS DVCAPGSITL DIIQQAEAEV KRLDQLKASR IKELFIKKQK 

       370        380        390        400        410        420 
ELEDTCNMSH METPSTEMGN ITNLVDSGEV DHVDLLAAMD EKIARAKEEA ASRKGIIEKV 

       430        440        450        460        470        480 
DRWMLASDEE RWLEEYDQDE NRYSVSRNAH RNLRRAERAR ITVSKISGLV ESILVKAKSW 

       490        500        510        520        530        540 
EVERQKVFLY NEVPLVAMLQ EYNKLRQEKE MEKQRLREMK KMSIPQPVAE GDNFYMARPA 

       550        560        570        580        590 
SSNRRISNRS MNGGFGSGSP INRKYSGGFN NTNNNYTALG TSIRRESRKS EA 

References and documentation are available.

Number of amino acids: 592

Molecular weight: 68293.71

Theoretical pI: 8.30

Amino acid composition: 
Ala (A)  37	  6.2%
Arg (R)  50	  8.4%
Asn (N)  31	  5.2%
Asp (D)  34	  5.7%
Cys (C)   5	  0.8%
Gln (Q)  20	  3.4%
Glu (E)  63	 10.6%
Gly (G)  27	  4.6%
His (H)  14	  2.4%
Ile (I)  36	  6.1%
Leu (L)  59	 10.0%
Lys (K)  50	  8.4%
Met (M)  18	  3.0%
Phe (F)  13	  2.2%
Pro (P)  13	  2.2%
Ser (S)  48	  8.1%
Thr (T)  21	  3.5%
Trp (W)   5	  0.8%
Tyr (Y)  16	  2.7%
Val (V)  32	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 97
Total number of positively charged residues (Arg + Lys): 100

Atomic composition:

Carbon      C	      2968
Hydrogen    H	      4832
Nitrogen    N	       876
Oxygen      O	       923
Sulfur      S	        23

Formula: C2968H4832N876O923S23
Total number of atoms: 9622

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    51590
Abs 0.1% (=1 g/l)   0.755, assuming all pairs of Cys residues form cystines


Ext. coefficient    51340
Abs 0.1% (=1 g/l)   0.752, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.64
This classifies the protein as unstable.



Aliphatic index: 84.51

Grand average of hydropathicity (GRAVY): -0.714