ProtParam
User-provided sequence:
10 20 30 40 50 60
MRNAGVQVDT NMQKISLQDT VLVNEMKGRV KFIGETQFAK GIWYGIELDK PLGKNDGSAN
70 80 90 100 110 120
GIRYFDIDLK KANSNGGYYG LFCKKDTLQF YKPDDDEHSL LNGNAAQETI KNLQVKCESL
130 140 150 160 170 180
ASKLNKIKIE NHELKTSVEK LSTNETVLLS KISRLDKLVK ELKVENGNMK THLDNFNHLL
190 200 210 220 230 240
DASDSVMAPD LDKGTLLERS HLLQGLLDQT KLSYDKAMKV QEDLLEENTQ LLEENAVLSK
250 260 270 280 290 300
KISDLGLQLQ QTNNTIGDLA LQIEAQSKSS NIVDKLTNDN ILLTSNIKAL NNELEELQAK
310 320 330 340 350 360
EKLDENLRIT YEQLEQELRL QLSNLQSALE NEKEIAGTYI EENSRLKATL ESIEAKTSHK
370 380 390 400 410 420
FQSLELKVNT LQEELYQNKL LKKFYQIYEP FAQPHLAALS SQLQYLAEVI ESENFGKLEN
430 440 450 460 470 480
IEIHIILKVL SSISYALHIY TIKNTPDHLE TTLQCFKVNI APISMWLSEF LQRKFSSKQE
490 500 510 520 530 540
TAFSICQFLE DNKFLDKDVT LILKILHPIL ETTVPKLLAF LRTNSNFNDN DTLCLIGSLY
550 560 570 580 590 600
ERSLSLIARI DKLIGKEEIS KQDNRLFLYP SCDITLSSIL TILFSDALFL RQDYKRISSL
610 620 630 640 650 660
KKLEVFFQGI ESLLENITIF PEQPSQQTSD SESQCNIKEG NFSNSLLSDR LNEENIRLKE
670 680 690 700 710 720
VLVQKENMLT ELETKIKIII GRDLERKTLE ENIKTLKVEL NNKNEENCGK TEILNKLKEE
730 740 750 760 770 780
NFNLVNRLKN MELKLYQIKD NNTLNKIYLD REKVDRVNLV SEIMELRETI RRQIKEQKRV
790 800 810 820 830 840
SIDFSWLDEL PAVENKQPFK EHINHSLDTL GIEMFNFVST SRILDLKLDQ PLAEDELWHE
850 860
RDHSYISYLK RKRKNIRLKS QNVVTYYK
References and
documentation are available.
Number of amino acids: 868
Molecular weight: 100289.71
Theoretical pI: 5.62
Total number of negatively charged residues (Asp + Glu): 133
Total number of positively charged residues (Arg + Lys): 116
Atomic composition:
Carbon C 4457
Hydrogen H 7225
Nitrogen N 1195
Oxygen O 1383
Sulfur S 19
Formula: C
4457H
7225N
1195O
1383S
19
Total number of atoms: 14279
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 56770
Abs 0.1% (=1 g/l) 0.566, assuming all pairs of Cys residues form cystines
Ext. coefficient 56270
Abs 0.1% (=1 g/l) 0.561, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 43.63
This classifies the protein as unstable.
Aliphatic index: 103.56
Grand average of hydropathicity (GRAVY): -0.484