ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRNFLVFSVI FIAYNSCEAA NIQADNTFAD PNIGEFRELL IDPKAGALFV GSEGAIFRLW 

        70         80         90        100        110        120 
AYNINDTGEN VFAKKQLVLS ESEESECRST ASDERLCRPS TRFLAFTNNL DSIYVCSSVG 

       130        140        150        160        170 
MRPEIRVLDS LSLRDQQEPR TEIGICVVDP TFNFTAVVVD SGNPEGKQVR SIDK 

References and documentation are available.

Number of amino acids: 174

Molecular weight: 19372.81

Theoretical pI: 4.62

Amino acid composition: 
Ala (A)  13	  7.5%
Arg (R)  12	  6.9%
Asn (N)  12	  6.9%
Asp (D)  11	  6.3%
Cys (C)   5	  2.9%
Gln (Q)   5	  2.9%
Glu (E)  14	  8.0%
Gly (G)   9	  5.2%
His (H)   0	  0.0%
Ile (I)  12	  6.9%
Leu (L)  13	  7.5%
Lys (K)   5	  2.9%
Met (M)   2	  1.1%
Phe (F)  12	  6.9%
Pro (P)   7	  4.0%
Ser (S)  16	  9.2%
Thr (T)   8	  4.6%
Trp (W)   1	  0.6%
Tyr (Y)   3	  1.7%
Val (V)  14	  8.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 17

Atomic composition:

Carbon      C	       852
Hydrogen    H	      1337
Nitrogen    N	       233
Oxygen      O	       269
Sulfur      S	         7

Formula: C852H1337N233O269S7
Total number of atoms: 2698

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    10220
Abs 0.1% (=1 g/l)   0.528, assuming all pairs of Cys residues form cystines


Ext. coefficient     9970
Abs 0.1% (=1 g/l)   0.515, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.09
This classifies the protein as unstable.



Aliphatic index: 86.84

Grand average of hydropathicity (GRAVY): -0.132