ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRNFSVFERF SKAFKDHPSL TRILVVSTIS GGGLIAYSEA NASYGANGGA VVETGTKKKK 

        70         80         90        100        110        120 
VVLLGTGWAG TSFLKNLNNS QYEVQIISPR NYFAFTPLLP SVTCGTVEAR SVVEPIRNIG 

       130        140        150        160        170        180 
RKNVDTSYLE AECFKIDPAS KKVYCRSKQG LSSNGKKEFS VDYDYLVIAT GAQSNTFNIP 

       190        200        210        220        230        240 
GVEENCHFLK EVEDAQRIRK TVIDSFEKAS LPELSDEERK RILHFVVVGG GPTGVEFAAE 

       250        260        270        280        290        300 
LHDFVTEDLV SLYPRAKGSV RITLLEAADH ILTMFDKRIT EFAEEKFSRD GIDVKLGSMV 

       310        320        330        340        350        360 
TKVNEKDISA KTKGGEVSSI PYGMIVWSTG IGTRPVIKDF MKQIGQGNRR ALATDEWLRV 

       370        380        390        400        410        420 
EGTDNIYALG DCATINQRKV MEDVSAIFSK ADKDKSGTLT LKEFQEAMDD ICVRYPQVEL 

       430        440        450        460        470        480 
YLKSKRMRGI ADLLKEAETD DVSKNNIELK IEEFKSALSQ VDSQVKFLPA TAQVAAQQGA 

       490        500        510        520        530        540 
YLAKCFDRME ECEKSPEGPI RMRGEGRHRF RPFRYRHLGQ FAPLGGEQTA AQLPGDWVSI 

       550        560        570        580 
GHSSQWLWYS VYASKQVSWR TRVLVVSDWM RRFIFGRDSS SI 

References and documentation are available.

Number of amino acids: 582

Molecular weight: 65059.09

Theoretical pI: 8.54

Amino acid composition: 
Ala (A)  42	  7.2%
Arg (R)  37	  6.4%
Asn (N)  19	  3.3%
Asp (D)  32	  5.5%
Cys (C)   8	  1.4%
Gln (Q)  20	  3.4%
Glu (E)  43	  7.4%
Gly (G)  44	  7.6%
His (H)   8	  1.4%
Ile (I)  34	  5.8%
Leu (L)  41	  7.0%
Lys (K)  43	  7.4%
Met (M)  11	  1.9%
Phe (F)  29	  5.0%
Pro (P)  19	  3.3%
Ser (S)  49	  8.4%
Thr (T)  31	  5.3%
Trp (W)   8	  1.4%
Tyr (Y)  17	  2.9%
Val (V)  47	  8.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 75
Total number of positively charged residues (Arg + Lys): 80

Atomic composition:

Carbon      C	      2893
Hydrogen    H	      4571
Nitrogen    N	       799
Oxygen      O	       869
Sulfur      S	        19

Formula: C2893H4571N799O869S19
Total number of atoms: 9151

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    69830
Abs 0.1% (=1 g/l)   1.073, assuming all pairs of Cys residues form cystines


Ext. coefficient    69330
Abs 0.1% (=1 g/l)   1.066, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.17
This classifies the protein as stable.



Aliphatic index: 80.89

Grand average of hydropathicity (GRAVY): -0.332