ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRPISLRLHE RPITQVLFNR EGDLLFVAAK DKLVSLWYTT NGERIGSYQC GGVVYSIDVS 

        70         80         90        100        110        120 
QDSKYLITAS ADAKARVWDV SSGRQLDSTD FEVSARSIEF SQGDKQILVV TDQVMGCQAK 

       130        140        150        160        170        180 
IHVFDFDKDE VRKLNKSYTL PSPQCKITQA TWGPLNKTIF ASCEDGAVRI YCTEKRELIK 

       190        200        210        220        230        240 
TILDHNKLVT RIEWTKHRIM FMTCSKDGTA KLYDTKTLKL LRTFDTGRPI NAAGISPLKP 

       250        260        270        280        290        300 
HVILGGGQSA ESVTTTKVDA SQFKVRFFHI VYGEELGGLI GHIGPVHSIC FTPDGKTFAT 

       310        320        330 
GGEEGLVQVN HLDESYFEFD DDLVYNPPVQ H 

References and documentation are available.

Number of amino acids: 331

Molecular weight: 37032.19

Theoretical pI: 6.56

Amino acid composition: 
Ala (A)  17	  5.1%
Arg (R)  16	  4.8%
Asn (N)   8	  2.4%
Asp (D)  24	  7.3%
Cys (C)   7	  2.1%
Gln (Q)  14	  4.2%
Glu (E)  17	  5.1%
Gly (G)  25	  7.6%
His (H)  10	  3.0%
Ile (I)  22	  6.6%
Leu (L)  27	  8.2%
Lys (K)  23	  6.9%
Met (M)   4	  1.2%
Phe (F)  16	  4.8%
Pro (P)  12	  3.6%
Ser (S)  23	  6.9%
Thr (T)  26	  7.9%
Trp (W)   4	  1.2%
Tyr (Y)  10	  3.0%
Val (V)  26	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 39

Atomic composition:

Carbon      C	      1654
Hydrogen    H	      2598
Nitrogen    N	       448
Oxygen      O	       495
Sulfur      S	        11

Formula: C1654H2598N448O495S11
Total number of atoms: 5206

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    37275
Abs 0.1% (=1 g/l)   1.007, assuming all pairs of Cys residues form cystines


Ext. coefficient    36900
Abs 0.1% (=1 g/l)   0.996, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.56
This classifies the protein as stable.



Aliphatic index: 85.65

Grand average of hydropathicity (GRAVY): -0.258