ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRPLYSCNLA TKDDIEMAGG VAPAHLEVNV GGYNTEQTIP IVKHQLVKVG RNDKECQLVL 

        70         80         90        100        110        120 
TNPSISSVHC VFWCVFFDED SIPMFYVKDC SLNGTYLNGL LLKRDKTYLL KHCDVIELSQ 

       130        140        150        160        170        180 
GSEENDIKKT RLVFMINDDL QSSLDPKLLD QMGFLREVDQ WEITNRIVGN GTFGHVLITH 

       190        200        210        220        230        240 
NSKERDEDVC YHPENYAVKI IKLKPNKFDK EARILLRLDH PNIIKVYHTF CDRNNHLYIF 

       250        260        270        280        290        300 
QDLIPGGDLF SYLAKGDCLT SMSETESLLI VFQILQALNY LHDQDIVHRD LKLDNILLCT 

       310        320        330        340        350        360 
PEPCTRIVLA DFGIAKDLNS NKERMHTVVG TPEYCAPEVG FRANRKAYQS FSRAATLEQR 

       370        380        390        400        410        420 
GYDSKCDLWS LGVITHIMLT GISPFYGDGS ERSIIQNAKI GKLNFKLKQW DIVSDNAKSF 

       430        440        450        460        470        480 
VKDLLQTDVV KRLNSKQGLK HIWIAKHLSQ LERLYYKKIL CNNEGPKLES INSDWKRKLP 

       490 
KSVIISQAIP KKKKVLE 

References and documentation are available.

Number of amino acids: 497

Molecular weight: 56849.61

Theoretical pI: 8.08

Amino acid composition: 
Ala (A)  19	  3.8%
Arg (R)  21	  4.2%
Asn (N)  29	  5.8%
Asp (D)  36	  7.2%
Cys (C)  14	  2.8%
Gln (Q)  19	  3.8%
Glu (E)  26	  5.2%
Gly (G)  26	  5.2%
His (H)  16	  3.2%
Ile (I)  38	  7.6%
Leu (L)  58	 11.7%
Lys (K)  44	  8.9%
Met (M)   8	  1.6%
Phe (F)  18	  3.6%
Pro (P)  18	  3.6%
Ser (S)  31	  6.2%
Thr (T)  21	  4.2%
Trp (W)   6	  1.2%
Tyr (Y)  17	  3.4%
Val (V)  32	  6.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 62
Total number of positively charged residues (Arg + Lys): 65

Atomic composition:

Carbon      C	      2546
Hydrogen    H	      4044
Nitrogen    N	       690
Oxygen      O	       739
Sulfur      S	        22

Formula: C2546H4044N690O739S22
Total number of atoms: 8041

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    59205
Abs 0.1% (=1 g/l)   1.041, assuming all pairs of Cys residues form cystines


Ext. coefficient    58330
Abs 0.1% (=1 g/l)   1.026, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.68
This classifies the protein as stable.



Aliphatic index: 97.83

Grand average of hydropathicity (GRAVY): -0.298