ProtParam
User-provided sequence:
10 20 30 40 50 60
MRPPVTGGRG GGGFRGGRDG GGRGFGGGRS FGGGRSGDRG RSGPRGRGRG APRGRGGPPR
70 80 90 100 110 120
GGMKGGSKVI VEPHRHAGVF IAKGKEDALV TKNLVPGEAV YNEKRISVQN EDGTKVEYRV
130 140 150 160 170 180
WNPFRSKLAA AILGGVDNIW IKPGAKVLYL GAASGTTVSH VSDLVGPEGC VYAVEFSHRS
190 200 210 220 230 240
GRDLVNMAKK RTNVIPIIED ARHPAKYRML VGMVDVIFSD VAQPDQARIL ALNASFFLKT
250 260 270 280 290 300
GGHFVISIKA NCIDSTVAAE AVFQSEVKKL QQEQFKPAEQ VTLEPFERDH ACVVGGYRMP
KKQKTPAS
References and
documentation are available.
Number of amino acids: 308
Molecular weight: 32829.53
Theoretical pI: 10.15
Total number of negatively charged residues (Asp + Glu): 28
Total number of positively charged residues (Arg + Lys): 48
Atomic composition:
Carbon C 1443
Hydrogen H 2316
Nitrogen N 444
Oxygen O 416
Sulfur S 9
Formula: C
1443H
2316N
444O
416S
9
Total number of atoms: 4628
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 20065
Abs 0.1% (=1 g/l) 0.611, assuming all pairs of Cys residues form cystines
Ext. coefficient 19940
Abs 0.1% (=1 g/l) 0.607, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.01
This classifies the protein as stable.
Aliphatic index: 72.14
Grand average of hydropathicity (GRAVY): -0.392