ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRPSTVTSRI WWSESSSSSS HDLSGSWEHT SLQRTSDHFS SMGSIDSLDH SSQLYPSGHL 

        70         80         90        100        110        120 
SSAKSNSSID HLGGHSKRDS AYGSFSTCSS TPDHTLPKAD ASSTENILYK VGLWEASRPG 

       130        140        150        160        170        180 
SSRQSQSTGD PQGLQDRPSS FLPRVPGNSS KSPRPEDNIE PKIATSGRSN FGPVWYVPDK 

       190        200        210        220        230        240 
KKAPSPPPLG LPLRSDSFAV AARGHEKARG PPFSDLASMQ HFTTLPHVQP RGDRRMETTD 

       250        260        270        280        290        300 
RQWKLAHPSS GKEIGNVGYQ PEGHLDCRWL CSDDRAGRPS GAPGRHQFSL SSTDVHFLKS 

       310        320        330        340        350        360 
YHGSQHPQPC SDESPRFPSS PRELLRITPS GCLQEPPELS QDDNPAQVRW PGSVNQKLDD 

       370        380        390        400        410        420 
RGRSHYFSVP HRQPVHGSAH VLIPRSDYWH SDTTPVDLEC PLLRPDQRGY PQQYEETPAS 

       430        440        450        460        470        480 
HERGGYQQLN AGIEGCCSGI HEPPRASHTV RAGLQCPGND FKLVDAESGR ISRQRTPMLH 

       490        500        510        520        530        540 
SLTQDGAWRP GNSKDCGNEK PPLLDAQVGK PTRRSDRFAT TLRNEIQMRR AKLQKSKSTV 

       550        560        570        580        590        600 
TLAGDSEAED CAGDWRVDVG AVPEGSFPST YKEHLKEAQT RVLKATSFQR RDLDPTPADQ 

       610        620        630        640        650        660 
YPGPPEHRTC DHSASSSLSS FPGEPDSAPR LCEAGLAKPP SSAGGVPHIL RIGGRKRFTA 

       670        680        690        700        710        720 
EQKLKSYSEP EKINEVGLSG DHSPHPTIRT SEDSVGTFAD RWKFFEETSK SLLQKPGHRQ 

       730        740        750        760        770        780 
AFCGIPREKA ERPQTQGHEC ESTEPWFQKR SRATSCGEIL SEDRKVEKAS EKLNPPRRLG 

       790        800        810        820        830        840 
TFAEYQASWK EQKKSLEARS SGRYHSADDI LDAGLDQQQR PQYIHERSRS SPSTDHYSQE 

       850        860        870        880        890        900 
VPVEPNRQAE DSGGQKEALL CTLQAEEGCS APSSSVLSSA QPQDSQHVNE DPTSPQPEAQ 

       910        920        930        940        950        960 
LSSKCQHLQT STMETSRSPS PQFAPQKLTD KPPLLIHEDN SARYCPVTAC YVFIPCRLQV 

       970        980        990       1000       1010       1020 
FVAGALPQLQ TLGIERVMDN NTTVKMVPIK IVHSESQPEK ESRQSLACPA ELPALPSGLE 

      1030       1040       1050       1060       1070       1080 
KDQIKTLSTS EQCYSRFCVY TRQEVETPHR ARPPEPQPPS TPAPPVRDSC SSPPSLNYGK 

      1090       1100       1110       1120       1130       1140 
AKEKTVDDLK SEELAREIVG KDKSLADILD PSVKIKTTMD LMEGIFPKDE YLLEEAQQRR 

      1150       1160       1170       1180       1190       1200 
KLLPKVPSPR VTEDNSCPSL SAICYNLGTR SKSVPVSQLI KIKILASSPR KQDPGMPGVV 

      1210       1220       1230       1240       1250       1260 
SLATNSTYYS TSAPKAELLI KMKDLQEPEE YSAGDLDHDL SIKKQELIDS ISRKLQVLRE 

      1270       1280       1290       1300       1310       1320 
ARESLLEDIQ ANNALGDEVE AIVKDVCKPN EFDKFRMFIG DLDKVVNLLL SLSGRLARVE 

      1330       1340       1350       1360       1370       1380 
NALNNLDDSP SPGDRQSLLE KQRVLTQQHE DAKELKENLD RRERIVFDIL ATYLSEENLA 

      1390       1400       1410       1420 
DYEHFVKMKS ALIIEQRELE DKIHLGEEQL KCLFDSLQPE RSK 

References and documentation are available.

Number of amino acids: 1423

Molecular weight: 157989.07

Theoretical pI: 6.32

Amino acid composition: 
Ala (A)  85	  6.0%
Arg (R)  98	  6.9%
Asn (N)  33	  2.3%
Asp (D)  90	  6.3%
Cys (C)  30	  2.1%
Gln (Q)  81	  5.7%
Glu (E) 106	  7.4%
Gly (G)  81	  5.7%
His (H)  46	  3.2%
Ile (I)  47	  3.3%
Leu (L) 123	  8.6%
Lys (K)  82	  5.8%
Met (M)  15	  1.1%
Phe (F)  34	  2.4%
Pro (P) 124	  8.7%
Ser (S) 176	 12.4%
Thr (T)  67	  4.7%
Trp (W)  14	  1.0%
Tyr (Y)  30	  2.1%
Val (V)  61	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 196
Total number of positively charged residues (Arg + Lys): 180

Atomic composition:

Carbon      C	      6836
Hydrogen    H	     10827
Nitrogen    N	      2019
Oxygen      O	      2203
Sulfur      S	        45

Formula: C6836H10827N2019O2203S45
Total number of atoms: 21930

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   123575
Abs 0.1% (=1 g/l)   0.782, assuming all pairs of Cys residues form cystines


Ext. coefficient   121700
Abs 0.1% (=1 g/l)   0.770, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 62.89
This classifies the protein as unstable.



Aliphatic index: 65.00

Grand average of hydropathicity (GRAVY): -0.827