ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRQKRAKSYR KQLLVYSHTF KFREPYQVLV DNQLVLECNN SNFNLPSGLK RTLQADVKVM 

        70         80         90        100        110        120 
ITQCCIQALY ETRNDGAINL AKQFERRRCN HSFKDPKSPA ECIESVVNIS GANKHRYVVA 

       130        140        150        160        170        180 
SQDIDLRRKL RTVPGVPLIH LTRSVMVMEP LSTASAKASK ITEEQKLYKG LNDPNIEKLQ 

       190        200        210        220        230        240 
ESGDGSGKES ITKKRKLGPK APNPLSVKKK KKVNSPSDEV KDKEDTSKEK KKRRRRKHKS 

       250 
NTNVPVSNGT TAAQ 

References and documentation are available.

Number of amino acids: 254

Molecular weight: 28801.17

Theoretical pI: 10.05

Amino acid composition: 
Ala (A)  14	  5.5%
Arg (R)  19	  7.5%
Asn (N)  17	  6.7%
Asp (D)  11	  4.3%
Cys (C)   5	  2.0%
Gln (Q)  12	  4.7%
Glu (E)  15	  5.9%
Gly (G)  10	  3.9%
His (H)   5	  2.0%
Ile (I)  10	  3.9%
Leu (L)  20	  7.9%
Lys (K)  34	 13.4%
Met (M)   4	  1.6%
Phe (F)   5	  2.0%
Pro (P)  13	  5.1%
Ser (S)  22	  8.7%
Thr (T)  13	  5.1%
Trp (W)   0	  0.0%
Tyr (Y)   6	  2.4%
Val (V)  19	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      1249
Hydrogen    H	      2084
Nitrogen    N	       384
Oxygen      O	       377
Sulfur      S	         9

Formula: C1249H2084N384O377S9
Total number of atoms: 4103

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     9190
Abs 0.1% (=1 g/l)   0.319, assuming all pairs of Cys residues form cystines


Ext. coefficient     8940
Abs 0.1% (=1 g/l)   0.310, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 57.09
This classifies the protein as unstable.



Aliphatic index: 73.27

Grand average of hydropathicity (GRAVY): -0.889