ProtParam
User-provided sequence:
10 20 30 40 50 60
MRQKRAKSYR KQLLVYSHTF KFREPYQVLV DNQLVLECNN SNFNLPSGLK RTLQADVKVM
70 80 90 100 110 120
ITQCCIQALY ETRNDGAINL AKQFERRRCN HSFKDPKSPA ECIESVVNIS GANKHRYVVA
130 140 150 160 170 180
SQDIDLRRKL RTVPGVPLIH LTRSVMVMEP LSTASAKASK ITEEQKLYKG LNDPNIEKLQ
190 200 210 220 230 240
ESGDGSGKES ITKKRKLGPK APNPLSVKKK KKVNSPSDEV KDKEDTSKEK KKRRRRKHKS
250
NTNVPVSNGT TAAQ
References and
documentation are available.
Number of amino acids: 254
Molecular weight: 28801.17
Theoretical pI: 10.05
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 53
Atomic composition:
Carbon C 1249
Hydrogen H 2084
Nitrogen N 384
Oxygen O 377
Sulfur S 9
Formula: C
1249H
2084N
384O
377S
9
Total number of atoms: 4103
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 9190
Abs 0.1% (=1 g/l) 0.319, assuming all pairs of Cys residues form cystines
Ext. coefficient 8940
Abs 0.1% (=1 g/l) 0.310, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 57.09
This classifies the protein as unstable.
Aliphatic index: 73.27
Grand average of hydropathicity (GRAVY): -0.889