ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRQLTEEETK VVFEKLAGYI GRNISFLVDN KELPHVFRLQ KDRVYYVPDH VAKLATSVAR 

        70         80         90        100        110        120 
PNLMSLGICL GKFTKTGKFR LHITSLTVLA KHAKYKIWIK PNGEMPFLYG NHVLKAHVGK 

       130        140        150        160        170        180 
MSDDIPEHAG VIVFAMNDVP LGFGVSAKST SESRNMQPTG IVAFRQADIG EYLRDEDTLF 


T 

References and documentation are available.

Number of amino acids: 181

Molecular weight: 20380.67

Theoretical pI: 9.28

Amino acid composition: 
Ala (A)  12	  6.6%
Arg (R)   9	  5.0%
Asn (N)   7	  3.9%
Asp (D)   9	  5.0%
Cys (C)   1	  0.6%
Gln (Q)   4	  2.2%
Glu (E)  10	  5.5%
Gly (G)  13	  7.2%
His (H)   7	  3.9%
Ile (I)  10	  5.5%
Leu (L)  17	  9.4%
Lys (K)  15	  8.3%
Met (M)   6	  3.3%
Phe (F)  10	  5.5%
Pro (P)   8	  4.4%
Ser (S)   9	  5.0%
Thr (T)  11	  6.1%
Trp (W)   1	  0.6%
Tyr (Y)   6	  3.3%
Val (V)  16	  8.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 24

Atomic composition:

Carbon      C	       923
Hydrogen    H	      1459
Nitrogen    N	       249
Oxygen      O	       257
Sulfur      S	         7

Formula: C923H1459N249O257S7
Total number of atoms: 2895

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.709, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.709, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.31
This classifies the protein as stable.



Aliphatic index: 90.44

Grand average of hydropathicity (GRAVY): -0.153