ProtParam
User-provided sequence:
10 20 30 40 50 60
MRQRVGRSIA RAKFINTALL GRKRPVMERV VDIAHVDSSK AIQPLMKELE TDTTEARYKV
70 80 90 100 110 120
LQSVLEIYDD EKNIEPALTK EFHKMYLDVA FEISLPPQMT ALDASQPWML YWIANSLKVM
130 140 150 160 170 180
DRDWLSDDTK RKIVDKLFTI SPSGGPFGGG PGQLSHLAST YAAINALSLC DNIDGCWDRI
190 200 210 220 230 240
DRKGIYQWLI SLKEPNGGFK TCLEVGEVDT RGIYCALSIA TLLNILTEEL TEGVLNYLKN
250 260 270 280 290 300
CQNYEGGFGS CPHVDEAHGG YTFCATASLA ILRSMDQINV EKLLEWSSAR QLQEERGFCG
310 320 330 340 350 360
RSNKLVDGCY SFWVGGSAAI LEAFGYGQCF NKHALRDYIL YCCQEKEQPG LRDKPGAHSD
370 380 390 400 410 420
FYHTNYCLLG LAVAESSYSC TPNDSPHNIK CTPDRLIGSS KLTDVNPVYG LPIENVRKII
430
HYFKSNLSSP S
References and
documentation are available.
Number of amino acids: 431
Molecular weight: 48189.94
Theoretical pI: 5.97
Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 2140
Hydrogen H 3341
Nitrogen N 579
Oxygen O 642
Sulfur S 23
Formula: C
2140H
3341N
579O
642S
23
Total number of atoms: 6725
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 67685
Abs 0.1% (=1 g/l) 1.405, assuming all pairs of Cys residues form cystines
Ext. coefficient 66810
Abs 0.1% (=1 g/l) 1.386, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.14
This classifies the protein as unstable.
Aliphatic index: 86.01
Grand average of hydropathicity (GRAVY): -0.260