ProtParam
User-provided sequence:
10 20 30 40 50 60
MRQVLQKDKR DVQLSKALSY LLRHTAVKEK LTIDSNGYTP LKELLSHNRL KTHKCTVDDI
70 80 90 100 110 120
HRIVKENDKQ RFHIKTLGAD EEWICATQGH SIKSIQPSDE VLVPITEASQ LPQELIHGTN
130 140 150 160 170 180
LQSVIKIIES GAISPMSRNH VHLSPGMLHA KGVISGMRSS SNVYIFIDCH SPLFFQTLKM
190 200 210 220 230
FRSLNNVYLS SSIPVELIQK VVVKGNLKDE EKLDTLRRIL HERNIPLEKI
References and
documentation are available.
Number of amino acids: 230
Molecular weight: 26196.41
Theoretical pI: 9.30
Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 32
Atomic composition:
Carbon C 1161
Hydrogen H 1906
Nitrogen N 332
Oxygen O 339
Sulfur S 8
Formula: C
1161H
1906N
332O
339S
8
Total number of atoms: 3746
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 11585
Abs 0.1% (=1 g/l) 0.442, assuming all pairs of Cys residues form cystines
Ext. coefficient 11460
Abs 0.1% (=1 g/l) 0.437, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 49.88
This classifies the protein as unstable.
Aliphatic index: 104.61
Grand average of hydropathicity (GRAVY): -0.317