ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRQVWFSWIV GLFLCFFNVS SAAQYEPPAT WENVDYKRTI DVSNAYISET IEITIKNIAS 

        70         80         90        100        110        120 
EPATEYFTAF ESGIFSKVSF FSAYFTNEAT FLNSQLLANS TTAPGDDGES EIRYGIIQFP 

       130        140        150        160        170        180 
NAISPQEEVS LVIKSFYNTV GIPYPEHVGM SEEQHLLWET NRLPLSAYDT KKASFTLIGS 

       190        200        210        220        230        240 
SSFEEYHPPN DESLLGKANG NSFEFGPWED IPRFSSNETL AIVYSHNAPL NQVVNLRRDI 

       250        260        270        280        290        300 
WLSHWASTIQ FEEYYELTNK AAKLSKGFSR LELMKQIQTQ NMRQTHFVTV LDMLLPEGAT 

       310        320        330        340        350        360 
DHYFTDLVGL VSTSHAERDH FFIRPRFPIF GGWNYNFTVG WTNKLSDFLH VSSGSDEKFV 

       370        380        390        400        410        420 
ASIPILNGPP DTVYDNVELS VFLPEGAEIF DIDSPVPFTN VSIETQKSYF DLNKGHVKLT 

       430        440        450        460        470 
FSYRNLISQV ANGQVLIKYD YPKSSFFKKP LSIACYIFTA LMGVFVLKTL NMNVTN 

References and documentation are available.

Number of amino acids: 476

Molecular weight: 54071.92

Theoretical pI: 5.01

Amino acid composition: 
Ala (A)  27	  5.7%
Arg (R)  13	  2.7%
Asn (N)  31	  6.5%
Asp (D)  20	  4.2%
Cys (C)   2	  0.4%
Gln (Q)  15	  3.2%
Glu (E)  33	  6.9%
Gly (G)  23	  4.8%
His (H)  11	  2.3%
Ile (I)  31	  6.5%
Leu (L)  39	  8.2%
Lys (K)  21	  4.4%
Met (M)   7	  1.5%
Phe (F)  38	  8.0%
Pro (P)  25	  5.3%
Ser (S)  47	  9.9%
Thr (T)  32	  6.7%
Trp (W)   9	  1.9%
Tyr (Y)  21	  4.4%
Val (V)  31	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      2481
Hydrogen    H	      3705
Nitrogen    N	       613
Oxygen      O	       729
Sulfur      S	         9

Formula: C2481H3705N613O729S9
Total number of atoms: 7537

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    80915
Abs 0.1% (=1 g/l)   1.496, assuming all pairs of Cys residues form cystines


Ext. coefficient    80790
Abs 0.1% (=1 g/l)   1.494, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.52
This classifies the protein as stable.



Aliphatic index: 81.91

Grand average of hydropathicity (GRAVY): -0.159