ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRRAFVERNT NETKISVAIA LDKAPLPEES NFIDELITSK HANQKGEQVI QVDTGIGFLD 

        70         80         90        100        110        120 
HMYHALAKHA GWSLRLYSRG DLIIDDHHTA EDTAIALGIA FKQAMGNFAG VKRFGHAYCP 

       130        140        150        160        170        180 
LDEALSRSVV DLSGRPYAVI DLGLKREKVG ELSCEMIPHL LYSFSVAAGI TLHVTCLYGS 

       190        200        210 
NDHHRAESAF KSLAVAMRAA TSLTGSSEVP STKGVL 

References and documentation are available.

Number of amino acids: 216

Molecular weight: 23519.82

Theoretical pI: 6.40

Amino acid composition: 
Ala (A)  25	 11.6%
Arg (R)  11	  5.1%
Asn (N)   6	  2.8%
Asp (D)  12	  5.6%
Cys (C)   3	  1.4%
Gln (Q)   4	  1.9%
Glu (E)  13	  6.0%
Gly (G)  16	  7.4%
His (H)  11	  5.1%
Ile (I)  13	  6.0%
Leu (L)  22	 10.2%
Lys (K)  11	  5.1%
Met (M)   5	  2.3%
Phe (F)   8	  3.7%
Pro (P)   6	  2.8%
Ser (S)  18	  8.3%
Thr (T)  11	  5.1%
Trp (W)   1	  0.5%
Tyr (Y)   6	  2.8%
Val (V)  14	  6.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	      1041
Hydrogen    H	      1651
Nitrogen    N	       293
Oxygen      O	       312
Sulfur      S	         8

Formula: C1041H1651N293O312S8
Total number of atoms: 3305

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    14565
Abs 0.1% (=1 g/l)   0.619, assuming all pairs of Cys residues form cystines


Ext. coefficient    14440
Abs 0.1% (=1 g/l)   0.614, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.75
This classifies the protein as stable.



Aliphatic index: 93.56

Grand average of hydropathicity (GRAVY): -0.054