ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MRRETVGEFS SDDDDDILLE LGTRPPRFTQ IPPSSAALQT QIPTTLEVTT TTLNNKQSKN 

        70         80         90        100        110        120 
DNQLVNQLNK AQGEASMLRD KINFLNIERE KEKNIQAVKV NELQVKHLQE LAKLKQELQK 

       130        140        150        160        170        180 
LEDEKKFLQM EARGKSKREV ITNVKPPSTT LSTNTNTITP DSSSVAIEAK PQSPQSKKRK 

       190        200        210        220        230        240 
ISDNLLKKNM VPLNPNRIIP DETSLFLESI LLHQIIGADL STIEILNRLK LDYITEFKFK 

       250        260        270        280        290        300 
NFVIAKGAPI GKSIVSLLLR CKKTLTLDRF IDTLLEDIAV LIKEISVHPN ESKLAVPFLV 

       310        320        330        340        350        360 
ALMYQIVQFR PSATHNLALK DCFLFICDLI RIYHHVLKVP IHESNMNLHV EPQIFQYELI 

       370        380        390        400        410        420 
DYLIISYSFD LLEGILRVLQ SHPKQTYMEF FDENILKSFE FVYKLALTIS YKPMVNVIFS 

       430        440        450        460        470        480 
AVEVVNIITS IILNMDNSSD LKSLISGSWW RDCITRLYAL LEKEIKSGDV YNENVDTTTL 

       490        500        510        520        530        540 
HMSKYHDFFG LIRNIGDNEL GGLISKLIYT DRLQSVPRVI SKEDIGMDSD KFTAPIIGYK 

       550        560        570        580        590        600 
MEKWLLKLKD EVLNIFENLL MIYGDDATIV NGEMLIHSSK FLSREQALMI ERYVGQDSPN 

       610        620        630        640        650        660 
LDLRCHLIEH TLTIIYRLWK DHFKQLREEQ IKQVESQLIM SLWRFLVCQT ETVTANEREM 

       670        680        690        700        710        720 
RDHRHLVDSL HDLTIKDQAS YYEDAFEDLP EYIEEELKMQ LNKRTGRIMQ VKYDEKFQEM 

       730        740 
ARTILESKSF DLTTLEEADS LYISMGL 

References and documentation are available.

Number of amino acids: 747

Molecular weight: 86424.81

Theoretical pI: 5.70

Amino acid composition: 
Ala (A)  28	  3.7%
Arg (R)  33	  4.4%
Asn (N)  38	  5.1%
Asp (D)  47	  6.3%
Cys (C)   6	  0.8%
Gln (Q)  34	  4.6%
Glu (E)  60	  8.0%
Gly (G)  21	  2.8%
His (H)  18	  2.4%
Ile (I)  68	  9.1%
Leu (L)  96	 12.9%
Lys (K)  58	  7.8%
Met (M)  21	  2.8%
Phe (F)  30	  4.0%
Pro (P)  25	  3.3%
Ser (S)  54	  7.2%
Thr (T)  44	  5.9%
Trp (W)   5	  0.7%
Tyr (Y)  22	  2.9%
Val (V)  39	  5.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 107
Total number of positively charged residues (Arg + Lys): 91

Atomic composition:

Carbon      C	      3878
Hydrogen    H	      6223
Nitrogen    N	      1017
Oxygen      O	      1154
Sulfur      S	        27

Formula: C3878H6223N1017O1154S27
Total number of atoms: 12299

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    60655
Abs 0.1% (=1 g/l)   0.702, assuming all pairs of Cys residues form cystines


Ext. coefficient    60280
Abs 0.1% (=1 g/l)   0.697, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 42.72
This classifies the protein as unstable.



Aliphatic index: 104.51

Grand average of hydropathicity (GRAVY): -0.255